RT info:eu-repo/semantics/article
T1 Immobilization of Rhodococcus by encapsulation and entrapment: a green solution to bitter citrus by-products
A1 Pilar Izquierdo, María Concepción
A1 López Fouz, María
A1 Ortega Santamaría, Natividad
A1 Busto Núñez, Mª Dolores
K1 Cell immobilization
K1 Ca-alginate hollow beads
K1 PVA/PEG cryogels
K1 Rhodococcus
K1 Debittering
K1 Citrus by-products
K1 Bioquímica
K1 Biochemistry
K1 Alimentos
K1 Food
AB Debittering of citrus by-products is required to obtain value-added compounds for application in the food industry (e.g., dietary fber, bioactive compounds). In this work, the immobilization of Rhodococcus fascians cells by encapsulation in Ca-alginate hollow beads and entrapment in poly(vinyl alcohol)/polyethylene glycol (PVA/PEG) cryogels was studied as an alternative to chemical treatments for degrading the bitter compound limonin. Previously, the Rhodococcus strain was adapted using orange peel extract to increase its tolerance to limonoids. The optimal conditions for the encapsulation ofmicrobial cells were 2% Na-alginate, 4% CaCl2, 4% carboxymethylcellulose (CMC), and a microbial load of 0.6 OD600 (optical density at 600 nm). For immobilization by entrapment, the optimal conditions were 8% PVA, 8% PEG, and 0.6 OD600 microbial load. Immobilization by entrapment protected microbial cells better than encapsulation against the citrus medium stress conditions (acid pH and composition). Thus, under optimal immobilization conditions, limonin degradation was 32 and 28% for immobilization in PVA/PEG gels and in hollow beads, respectively, in synthetic juice (pH 3) after 72 h at 25 °C. Finally, the microbial cells entrapped in the cryogels showed a higher operational stability in orange juice than the encapsulated cells, with four consecutive cycles of reuse (runs of 24 h at 25 °C).
PB Springer Nature
SN 0175-7598
YR 2023
FD 2023-08
LK http://hdl.handle.net/10259/8192
UL http://hdl.handle.net/10259/8192
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 08-may-2024