RT info:eu-repo/semantics/article T1 Barley seed coating with urease and phosphatase to improve N and P uptake A1 Mvila, Beaufray Gilaime A1 Pilar Izquierdo, María Concepción A1 Busto Núñez, Mª Dolores A1 Pérez Mateos, Manuel A1 Ortega Santamaría, Natividad K1 Soil enzymes K1 Seed encapsulation K1 Plant nutrients uptake K1 Humic acids K1 Bioquímica K1 Biochemistry K1 Alimentos K1 Food AB In this work, barley seed coating with urease and phosphatase, free and immobilized in humic acids, was studied as a tool to enhance the use efficiency of N and P by plants. The seeds were encapsulated in gels of Ca-alginate by a method that allows gelation of the polymer, containing enzymes, from the surface of seeds outward. The coating process did not affect seed germination. A pot experiment with the coated seeds was carried out. The seed coating with free enzymes increased soil ammonium-N (NH4+-N) and inorganic P up to 39 and 54 %, respectively, and up to 62 and 64 %, with the immobilized enzymes, in comparison with control pots. Seed coating improved N (47-137 %) and P (29-61 %) uptake by plants. Moreover, a synergistic response in N and P uptake with both urease and phosphatase was observed. It can be concluded that the seed coating with urease and phosphatase exerted a positive effect enhancing the use of N and P by plants, which could lead to the development of natural fertilizers, minimizing the application of chemical fertilizers. PB Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz YR 2020 FD 2020-01 LK http://hdl.handle.net/10259/8193 UL http://hdl.handle.net/10259/8193 LA eng DS Repositorio Institucional de la Universidad de Burgos RD 09-may-2024