RT info:eu-repo/semantics/article
T1 Synthesis and characterization of a stable humic–urease complex: application to barley seed encapsulation for improving N uptake
A1 Mvila, Beaufray Gilaime
A1 Pilar Izquierdo, María Concepción
A1 Busto Núñez, Mª Dolores
A1 Pérez Mateos, Manuel
A1 Ortega Santamaría, Natividad
K1 Enzyme immobilization
K1 Nitrogen availability
K1 Plant growth
K1 Seed coating
K1 Seed encapsulation
K1 Soil urease
K1 Bioquímica
K1 Biochemistry
K1 Agricultura
K1 Agriculture
AB BACKGROUNDMost N fertilizers added to soil are not efficiently used by plants and are lost to the atmosphere or leached from the soil, causing environmental pollution and increasing cost. Barley seed encapsulation in calcium alginate gels containing free or immobilized urease to enhance plant utilization of soil N was investigated.RESULTSUrease was immobilized with soil humic acids (HA). A central composite face-centered design was applied to optimize the immobilization process, reaching an immobilization yield of 127%. Soil stability of urease was enhanced after the immobilization. Seed encapsulation with free urease (FU) and humic–urease complex (HUC) resulted in a urease activity retention in the coating layer of 46% and 24%, and in germination rates of 87% and 92%, respectively. Under pot culture conditions, the pots planted with seeds encapsulated with FU and HUC showed higher ammonium N (NH4+-N) (26% and 64%, respectively) than the control soil at 28 days after planting (DAP). Moreover, the seed encapsulation with FU and HUC increased the N uptake 83% and 97%, respectively, at 35 DAP.CONCLUSIONSeed encapsulation with urease could substantially contribute to enhancing plant N nutrition in the early stages of seedling establishment.
PB Society of Chemical Industry
SN 0022-5142
YR 2015
FD 2015-09
LK http://hdl.handle.net/10259/8264
UL http://hdl.handle.net/10259/8264
LA eng
NO We thank the ‘Ministere de L’Enseignement Superieur de laRepublique du Congo’ for a fellowship awarded to BG Mvila.
DS Repositorio Institucional de la Universidad de Burgos
RD 24-dic-2024