RT info:eu-repo/semantics/article
T1 Streptomyces tsukubaensis as a new model for carbon repression: transcriptomic response to tacrolimus repressing carbon sources
A1 Ordóñez Robles, María
A1 Santos Beneit, Fernando
A1 Albillos García, Silvia María
A1 Liras, Paloma
A1 Martín, Juan F.
A1 Rodríguez García, Antonio
K1 Streptomycestsukubaensis
K1 Tacrolimus
K1 FK506
K1 Carbon regulation
K1 Transcriptomics
K1 Microbiología
K1 Microbiology
K1 Biotecnología
K1 Biotechnology
K1 Biología
K1 Biology
AB In this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcriptomic profiling of S. tsukubaensis growing under tacrolimus producing and non-producing conditions. The analysis revealed important and different metabolic changes after the additions and a lack of transcriptional activation of the fkb cluster. In addition, we detected important differences in the transcriptional response to glucose between S. tsukubaensis and the model species Streptomyces coelicolor. A number of genes encoding key players of morphological and biochemical differentiation were strongly and permanently downregulated by the carbon sources. Finally, we identified several genes showing transcriptional profiles highly correlated to that of the tacrolimus biosynthetic pathway regulator FkbN that might be potential candidates for the improvement of tacrolimus production.
PB Springer
SN 0175-7598
YR 2017
FD 2017
LK http://hdl.handle.net/10259/8308
UL http://hdl.handle.net/10259/8308
LA eng
NO This work was supported by the European Union through an ERA-IB (PIM2010EEI-00677) international cooperation project. M. Ordóñez-Robles received a FPU fellowship of the Ministerio de Educación y Ciencia (Spain).
DS Repositorio Institucional de la Universidad de Burgos
RD 08-may-2024