RT info:eu-repo/semantics/article
T1 The different anion transport capability of prodiginine- and tambjamine-like molecules
A1 Fiore, Michele
A1 García Valverde, María
A1 Carreira Barral, Israel
A1 Moran, Óscar
K1 Anionophore
K1 Anion transport
K1 Anion binding
K1 Prodiginine
K1 Tambjamine
K1 Química orgánica
K1 Chemistry, Organic
AB Prodiginines and tambjamines are anion-selective ionophores capable of facilitating the transport of anions across the plasma membrane in mammalian cells. One of the potential applications of these anionophores is the possibility of employing them as a substitutive therapy for pathologies involving anion channels, as in cystic fibrosis. We have studied the interaction of a large anion as gluconate with three prodiginine- and two tambjamine-like compounds. Apparent dissociation constants for the chloride, iodide and gluconate complexes were estimated from iodide influx experiments in mammalian cells exposed to different extracellular anion combinations. Our experiments indicate that gluconate is not transported by the prodiginines, leaving the anionophores free to transport chloride and iodide. Conversely, gluconate would be transported to some extent by the tambjamines, competing with halides for the anionophores, and consequently reducing their flux. This might be related to the different structural features of both families of compounds. These data have important implications for the selection of impermeable anions in the analysis of the anionophore mechanism.
PB Elsevier
SN 0014-2999
YR 2020
FD 2020
LK http://hdl.handle.net/10259/8328
UL http://hdl.handle.net/10259/8328
LA eng
NO This work has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 667079.
DS Repositorio Institucional de la Universidad de Burgos
RD 04-dic-2024