RT info:eu-repo/semantics/article
T1 Influenza B Virus NS1-Truncated Mutants: Live-Attenuated Vaccine Approach
A1 Hai, Rong
A1 Martínez Sobrido, Luis
A1 Fraser, Kathryn A.
A1 Ayllón Barasoain, Juan
A1 García Sastre, Adolfo
A1 Palese, Peter
K1 Microbiología
K1 Microbiology
K1 Medicina
K1 Medicine
K1 Biología molecular
K1 Molecular biology
AB Type B influenza viruses can cause substantial morbidity and mortality in the population, and vaccinationremains by far the best means of protection against infections with these viruses. Here, we report theconstruction of mutant influenza B viruses for potential use as improved live-virus vaccine candidates.Employing reverse genetics, we altered the NS1 gene, which encodes a type I interferon (IFN) antagonist. Theresulting NS1 mutant viruses induced IFN and, as a consequence, were found to be attenuated in vitro and invivo. The absence of pathogenicity of the NS1 mutants in both BALB/c and C57BL/6 PKR / mice wasconfirmed. We also provide evidence that influenza B virus NS1 mutants induce a self-adjuvanted immuneresponse and confer effective protection against challenge with both homologous and heterologous B virusstrains in mice.
PB American Society for Microbiology
SN 0022-538X
YR 2008
FD 2008-11
LK http://hdl.handle.net/10259/8377
UL http://hdl.handle.net/10259/8377
LA eng
NO We  thank  Mark  A.  Yondola  for  helpful  discussions  and  criticalreviewing.  We  also  express  our  appreciation  to  Christopher  Narbus,Lily Ngai, and Richard Cadagan for excellent technical assistance.This work was supported by grants from the NIH, R01 AI46954 (toA.G.-S.),  U01  AI70469,  and  U19  AI62623  (Center  for  InvestigatingViral Immunity and Antagonism), and by CRIP (Center for Researchon  Influenza  Pathogenesis,  NIAID  contract  HHSN266200700010C).K.A.F. was supported by NIH training grant T32 AI007647.
DS Repositorio Institucional de la Universidad de Burgos
RD 24-nov-2024