RT info:eu-repo/semantics/article T1 The Impact of In Vitro Digestion on the Polyphenol Content and Antioxidant Activity of Spanish Ciders A1 Cavia Camarero, María del Mar A1 Arlanzón, Nerea A1 Busto Vázquez, Natalia A1 Carrillo Pérez, Celia A1 Alonso de la Torre, Sara K1 Apple wine K1 Digestion protocols K1 Antioxidants K1 DPPH K1 ABTS K1 FRAP K1 ORAC K1 Biología molecular K1 Molecular biology K1 Alimentos K1 Food AB Various factors can influence the polyphenol content and the antioxidant capacity of ciders,such as the apple variety, its degree of maturity, apple farming and storage conditions, and thecider-fermentation method, all of which explains why ciders of different origin present differentvalues. In addition, digestive processes could have some effects on the properties of cider. Hence,the objective of this study is to characterize Spanish ciders in terms of their polyphenol contentand antioxidant capacity and to ascertain whether those same properties differ in digested ciders.In total, 19 ciders were studied from three different zones within Spain: Asturias (A) (10), theBasque Country (BC) (6), and Castile-and-Leon (CL) (3). A range of assays was used to determinethe total polyphenol content and the antioxidant capacity of the ciders. In addition, a digestiveprocess was simulated in vitro, assessing whether the use of amylase might influence the recovery ofbioactive compounds after digestion. The Basque Country ciders presented higher total polyphenolcontents (830 ± 179 GAE/L) and higher antioxidant capacities (DPPH: 5.4 ± 1.6 mmol TE/L; ABTS:6.5 ± 2.0 mmol TE/L; FRAP: 6.9 ± 1.6 mmol TE/L) than the other ciders that were studied. Thein vitro digestion process, regardless of the use of amylase, implied a loss of phenolic compounds(598 ± 239 mg GAE/L undigested samples; 466 ± 146 mg GAE/L digested without amylase samples;420 ± 115 mg GAE/L digested with amylase samples), although the variation in antioxidant activitydepended on the assay chosen for its determination. PB MDPI YR 2023 FD 2023-04 LK http://hdl.handle.net/10259/8414 UL http://hdl.handle.net/10259/8414 LA eng DS Repositorio Institucional de la Universidad de Burgos RD 09-may-2024