RT info:eu-repo/semantics/article
T1 Immobilization of naringinase on asymmetric organic membranes: Application for debittering of grapefruit juice
A1 González Temiño, Yaiza
A1 Ruiz Pérez, María Olga
A1 Ortega Santamaría, Natividad
A1 Ramos Gómez, Sonia
A1 Busto Núñez, Mª Dolores
K1 Enzymatic membrane reactor
K1 Naringinase
K1 Polyethersulfone membrane
K1 Crosslinking
K1 Debittering
K1 Grapefruit juice
K1 Bioquímica
K1 Biochemistry
K1 Alimentos
K1 Food
K1 Ingeniería química
K1 Chemical engineering
AB An enzymatic membrane reactor (EMR) is assembled for the immobilization of naringinase on a polyethersulfone ultrafiltration membrane, based on fouling-induced method. The effects of molecular weight cut-off, membrane configuration, applied pressure, enzyme concentration and pH are studied in terms of permeate rate, immobilization efficiency, and biocatalytic conversion. The 10 kDa membrane operating in reverse mode, 0.2 MPa, 0.3 gL−1 of enzyme in acetate buffer at pH 5 and cross-linking with 0.25% glutaraldehyde showed the highest naringin conversion (73%). It was determined that the intermediate pore blocking model was the predominant fouling mechanism for the enzymatic immobilization. The EMR was applied for debittering of grapefruit juice, achieving a conversion of naringin below bitterness threshold and maintaining the antioxidant capacity of the juice. Furthermore, the biocatalytic activity of immobilized enzyme was retained at a high level at least during three consecutive reaction runs, with overnight storage at 4 °C after each run.
PB Elsevier
SN 1466-8564
YR 2021
FD 2021-10
LK http://hdl.handle.net/10259/8551
UL http://hdl.handle.net/10259/8551
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 08-may-2024