RT info:eu-repo/semantics/article
T1 Penicillium chrysogenum as a fungal factory for feruloyl esterases
A1 García-Calvo, Laura
A1 Rodríguez-Castro, Raquel
A1 Ullán, Ricardo V.
A1 Albillos García, Silvia María
A1 Fernández-Aguado, Marta
A1 Vicente, Cláudia M.
A1 Degnes, Kristin F.
A1 Sletta, Sletta
A1 Barreiro, Carlos
K1 Fungi
K1 Penicillium rubens
K1 Penicillium chrysogenum
K1 Lignocellulose
K1 Feruloyl esterase
K1 Extracellular proteome
K1 Bioquímica
K1 Biochemistry
K1 Biología molecular
K1 Molecular biology
AB Plant biomass is a promising substrate for biorefinery, as well as a source of bioactive compounds, platform chemicals, and precursors with multiple industrial applications. These applications depend on the hydrolysis of its recalcitrant structure. However, the effective biological degradation of plant cell walls requires several enzymatic groups acting synergistically, and novel enzymes are needed in order to achieve profitable industrial hydrolysis processes. In the present work, a feruloyl esterase (FAE) activity screening of Penicillium spp. strains revealed a promising candidate (Penicillium rubens Wisconsin 54–1255; previously Penicillium chrysogenum), where two FAE-ORFs were identified and subsequently overexpressed. Enzyme extracts were analyzed, confirming the presence of FAE activity in the respective gene products (PrFaeA and PrFaeB). PrFaeB-enriched enzyme extracts were used to determine the FAE activity optima (pH 5.0 and 50–55 °C) and perform proteome analysis by means of MALDI-TOF/TOF mass spectrometry. The studies were completed with the determination of other lignocellulolytic activities, an untargeted metabolite analysis, and upscaled FAE production in stirred tank reactors. The findings described in this work present P. rubens as a promising lignocellulolytic enzyme producer.
PB Springer
SN 0175-7598
YR 2023
FD 2023-02
LK http://hdl.handle.net/10259/8596
UL http://hdl.handle.net/10259/8596
LA eng
NO This study was funded by a project (ref. no. CTM2012-32026) of the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) and co-funded by the ERDF (European Regional Development Fund). Ms. Laura García-Calvo was supported by a PhD grant (ref. no. BES-2013–064578) and an international stay grant (ref. no. EEBB-I-16–11760), awarded by the Spanish Ministry MINECO.
DS Repositorio Institucional de la Universidad de Burgos
RD 09-may-2024