RT info:eu-repo/semantics/article
T1 Onion (Allium cepa L.) Skin Waste Valorization: Unveiling the Phenolic Profile and Biological Potential for the Creation of Bioactive Agents through Subcritical Water Extraction
A1 Trigueros Andrés, Ester
A1 Benito Román, Oscar
A1 Oliveira, Andreia P.
A1 Videira, Romeu A.
A1 Andrade, Paula B.
A1 Sanz Díez, Mª Teresa
A1 Beltrán Calvo, Sagrario
K1 Hydrothermal treatment
K1 Onion waste
K1 Polyphenols
K1 Quercetin
K1 Antioxidant activity
K1 Antidiabetic potential
K1 Ingeniería química
K1 Chemical engineering
AB Onion skin waste (OSW), the primary non-edible byproduct from onion processing, offers a renewable source of bioactive compounds. This study aims to valorize OSW through subcritical water extraction (SWE), aligning with a circular economy and biorefinery principles. SWE was carried out at 145 °C and 50 bar for 50 min in a discontinuous reactor, producing a phenolic-rich extract (32.3 ± 2.6 mg/g) dominated by protocatechuic acid (20.3 ± 2.5 mg/g), quercetin-4′-O-glucoside (7.5 ± 0.2 mg/g), and quercetin (3.2 ± 0.6 mg/g). Additionally, the extract contains sugars (207.1 ± 20.3 mg sucrose-Eq/g), proteins (22.8 ± 1.6 mg BSA-Eq/g), and free amino acids (20.4 ± 1.2 mg arginine-Eq/g). Its phenolic richness determines its scavenging activity against ●NO and O2●− radicals and its α-glucosidase and aldose-reductase inhibition without affecting α-amylase. Notably, the extract demonstrates significant α-glucosidase inhibition (IC50 = 75.6 ± 43.5 µg/mL), surpassing acarbose (IC50 = 129.5 ± 1.0 µg/mL) in both pure enzyme and cell culture tests without showing cytotoxicity to AGS, HepG2, and Caco-2 human cell lines. The extract’s bioactivity and nutritional content make it suitable for developing antioxidant and antidiabetic nutraceutical/food components, highlighting SWE’s potential for OSW valorization without using organic solvents.
PB MDPI
SN 2076-3921
YR 2024
FD 2024-02
LK http://hdl.handle.net/10259/8778
UL http://hdl.handle.net/10259/8778
LA eng
NO This research was funded by Portuguese national funds (Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through the project UIDP/50006/2020 of REQUIMTE/LAQV, and by the Spanish Agencia Estatal de Investigación [grant number PID2020-116716RJ-I00/AEI/10.13039/501100011033].
DS Repositorio Institucional de la Universidad de Burgos
RD 09-may-2024