RT info:eu-repo/semantics/bachelorThesis
T1 Estudio de recuperación de ácido galacturónico de residuos de piel de cebolla mediante un proceso combinado de extracción con agua subcrítica y diafiltración
A1 Tejera Ramos, Laura Inés
A2 Universidad de Burgos. Departamento de Biotecnología y Ciencia de los Alimentos
K1 Oligosaccharide fractionation
K1 Membrane fouling
K1 Solid byproduct valorisation
K1 Biorefinery
K1 Ingeniería química
K1 Chemical engineering
K1 Alimentos
K1 Food
AB In this work the feasibility of a diafiltration process using tubular ceramic membranes for the recovery of galacturonic acid, extracted from onion skin wastes, has been studied.In the first stage of the work, galacturonic acid (which is the main component of pectin) was extracted from onion skin wastes (OSW) using subcritical water. In each experiment, 15 g of OSW were mixed with 0,35 L of water in a high-pressure extractor and heated up to 145ºC and 40 bar for 45 min. The liquid extract was characterized in terms of composition by HPLC, in order to quantify the amount of galacturonic acid extracted (either in monomeric or oligomeric form) and the impurities co-extracted: free sugars (monosaccharides), organic acids (mainly formic and acetic) and other degradation products such as furfural and HMF formed from the degradation of the sugars present in the raw material.In the second stage of this work the aim was to study the separation of the oligomeric galacturonic acid from these impurities. For that purpose, multichannel ceramic membranes (Tami Industries) with an active layer of TiO2 and 132 cm2of effective area were used. The molecular weight cut off (MWCO) of the selected membranes were 1, 5 and 50 kDa. Feed flowrate was set at 15 L/h and transmembrane pressure (TMP, in the range from 0.2 to 1.25 bar) was adjusted to the desired values by using a valve placed after the membrane module. Permeate fluxes (Jp) were determined volumetrically under different operating conditions. The retention of galacturonic acid (either in monomeric or oligomeric form were calculated), as well as that of the impurities was calculated. All the experiments were carried out at 25 ºC and in total recirculation mode.It was found that the higher the MWCO, the higher the permeate flow rate, leading to a final flux of permeate constant due to the high degree of fouling observed on the membrane. It was also observed a dramatic decrease in the permeate flux when using the subcritical water extract (< 90 %) compared to that obtained when distilled water was used. This fact indicated an important membrane fouling due to the presence of colloidal matter. This fouling resulted to be reversible, since after chemical cleaning, the membrane recovered the full filtration capacity, evaluated in terms of permeate flux using water. All in all, a retention of oligomeric galacturonic acid higher than 70% was obtained when the MWCO was1 kDa, which was reduced to 50 % when either the 5 or 50 kDa membranes were used.Then, in order to complete the separation of the oligomeric galacturonic acid and the low molecular weight purities a diafiltration process using the 50 kDa membrane was carried out. After 480 min of experiment the complete removal of the impurities was obtained, leading to a retentate rich in oligomeric galacturonic acid.
LK http://hdl.handle.net/10259/9141
UL http://hdl.handle.net/10259/9141
LA spa
NO El trabajo presentado en esta memoria de TFG ha sido desarrollado en el seno delGrupo de Investigación Biotecnología Industrial y Medioambiental, reconocido por laUBU (GIR-UBU BIOIND) y por la Junta de Castilla y León como Unidad de InvestigaciónConsolidada UIC-128, en el marco de los siguientes proyectos de investigación:PID2019-104950RB-I00 - Valorización de subproductos marinos mediante tecnologíasde fluidos sub- y supercríticos para la obtención de biocompuestos valiosos, financiadopor la Agencia Estatal de Investigación.BU050P20 - Valorización de la fracción proteica de subproductos de la industriaagroalimentaria de Castilla y León mediante tecnologías de fluidos presurizados,financiado por la Junta de Castilla y León y el Fondo Europeo de Desarrollo Regional(FEDER).PID2020-116716RJ-I00 - Tecnologías de fluidos presurizados para la valorización desubproductos de la industria agroalimentaria: recuperación de pectina y otroscompuestos bioactivos de alto valor, financiado por la Agencia Estatal de Investigación.
DS Repositorio Institucional de la Universidad de Burgos
RD 08-ene-2025