RT info:eu-repo/semantics/article T1 Potential of Subcritical Water Hydrolysis to Valorize Low-Valued Ray-Finned Fish (Labeobarbus nedgia): Effects of Hydrolysis Temperature and Pressurization Agent A1 Tadesse, Solomon Abebaw A1 Emire, Shimelis Admassu A1 Barea Gómez, Pedro A1 Illera Gigante, Alba Ester A1 Melgosa Gómez, Rodrigo A1 Beltrán Calvo, Sagrario A1 Sanz Díez, Mª Teresa K1 Low valued ray-finned fish K1 Subcritical water hydrolysis K1 Nitrogen K1 Carbon dioxide K1 Degree of hydrolysis K1 Pressurization agent K1 Ingeniería química K1 Chemical engineering K1 Alimentos K1 Food K1 Biotecnología K1 Biotechnology AB Subcritical water (SCW) hydrolysis was applied to valorize the low-valued ray-finned fish (Labeobarbus nedgia) into valuable protein hydrolysates, employing N2 and CO2 as pressurization agents at varying temperatures (140, 160, 180, and 200 °C). The degree of hydrolysis (DH) and total free amino acid content increased with temperature for both pressurizing agents. The highest DH (54.5 ± 0.4%) and total free amino acid content (210 ± 1 mg/gprot) were observed at 200 °C when CO2 gas was used as the pressurizing agent. Predominantly, glycine and alanine were released for both pressurizing agents. The antioxidant activity, evaluated through three different assays, increased with temperature and was found to be the highest at 200 °C. This study illustrated the advantages of the intensified SCW technology by using CO2 as a pressurization agent in valorizing low-valued ray-finned fish (Labeobarbus nedgia), as animal residue rich in proteins, for the production of valuable protein hydrolysates with a high fraction of valuable free amino acids, which could offer potential applications as a functional ingredient in the food industry. PB MDPI SN 2304-8158 YR 2024 FD 2024-05-09 LK http://hdl.handle.net/10259/9181 UL http://hdl.handle.net/10259/9181 LA eng NO This work was funded by the Agencia Estatal de Investigación (AEI) and Ministerio de Ciencia e Innovación (MICINN) [grant number PID2022-136385OB-I00], by the AEI, MICINN, UE NextGenerationEU (Plan de Recuperación, Transformación y Resiliencia) [grant numbers TED2021-129311B-I00 and PDC2022-133443-I00] and by the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [grant number BU027P23]. The Pedro Barea predoctoral contract was funded by JCyL and the European Social Fund (ESF) by ORDEN EDU/1868/2022, de 19 de diciembre. Alba Ester Illera post-doctoral contracts were funded by JCyL and ERDF through project BU027P23. Rodrigo Melgosa contract was funded by a Beatriz Galindo Research Fellowship [BG20/00182]. DS Repositorio Institucional de la Universidad de Burgos RD 28-jun-2024