RT info:eu-repo/semantics/bookPart
T1 Hydrolysis of Lactose: Conventional Techniques and Enzyme Immobilization Strategies on Polymeric Supports
A1 Vallejo García, Jorge Lucas
A1 Cutillo Foraster, Alessandra
A1 Arnáiz Alonso, Ana
A1 Vallejos Calzada, Saúl
A1 García Pérez, José Miguel
A1 Muñoz Santamaría, María Asunción
A1 Trigo López, Miriam
K1 Diary products
K1 Lactose hydrolysis
K1 Enzyme immobilization
K1 Advanced polymers
K1 Polymeric supports
K1 Química orgánica
K1 Chemistry, Organic
K1 Alimentos
K1 Food
K1 Polímeros
K1 Polymers
AB This chapter explores lactose hydrolysis, emphasizing conventional techniquesand the noteworthy immobilization of β-galactosidase on polymeric matrices toenhance the process. Lactose, present in milk and dairy, poses challenges for lactoseintolerantindividuals, requiring enzymatic hydrolysis for lactose-free product development.The presence of other milk components, such as proteins and minerals, canindirectly influence the efficiency of lactose hydrolysis by potentially interacting withβ-galactosidase enzyme or affecting its stability and activity, making it necessary tocontrol factors such as enzyme concentration, temperature, pH, and reaction time toimprove lactose hydrolysis rates. The chapter delves into established methodologies,covering enzymatic kinetics, reaction conditions, and substrate concentrations. Italso describes the innovative approach of immobilizing β-galactosidase on polymericsupports to enhance enzyme stability, reusability, and overall efficiency in lactosehydrolysis. Discussions include the design of suitable polymeric matrices, providinginsights into mechanisms governing catalytic performance. This comprehensiveexploration contributes to understanding lactose hydrolysis, offering valuable insightsfor developing efficient and sustainable enzymatic processes applicable to the foodand pharmaceutical industries.
PB IntechOpen
YR 2024
FD 2024-05-22
LK http://hdl.handle.net/10259/9192
UL http://hdl.handle.net/10259/9192
LA eng
NO We gratefully acknowledge the financial support provided by all funders. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author Saul Vallejos received grant BG22/00086 funded by the Spanish Ministerio de Universidades. Author Jose Miguel García received grant PID2020-113264RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe,” The financial support provided by Fondo Europeo de Desarrollo Regional-European Regional Development Fund (FEDER, ERDF) and Regional Government of Castilla y León -Consejería de Educación, Junta de Castilla y León- (BU025P23) is gratefully acknowledged.
DS Repositorio Institucional de la Universidad de Burgos
RD 17-jul-2024