RT info:eu-repo/semantics/article
T1 Phase variation modulates the multi-phenotypes displayed by clinical Campylobacter jejuni strains
A1 Ortega Sanz, Irene
A1 Bocigas, Carolina
A1 Melero Gil, Beatriz
A1 Rovira Carballido, Jordi
K1 Campylobacteriosis
K1 Aerotolerance
K1 Oxidative stress
K1 Motility
K1 Biofilm formation
K1 Genomics
K1 Biotecnología
K1 Biotechnology
K1 Alimentos
K1 Food
AB The high incidence and prevalence of Campylobacter spp. in the food supply chain entail the importance to understand their mechanisms developed to withstand harsh environmental conditions encountered. Different stress conditions and phenotypic approaches were evaluated to study the behaviour of five clinical C. jejuni isolates with different genotypes, including the tolerance to oxygen and the oxidants hydrogen peroxide and cumene hydroperoxide, the motility and the ability to form biofilm on polystyrene and stainless steel at different temperatures and atmospheres. Whole Genome Sequencing was performed to analyse the occurrence of 216 genes involved in these mechanisms plus phase variation. The isolates showed high tolerance to oxygen and peroxide stress with different swimming motility performances and biofilm formation abilities. Aerotolerance was related with a reduced sensitive to peroxide stress and a loss of motility that promotes biofilm formation depending on the material surface. Comparative genomics did not reveal any clear gene pattern, although phase variation occurring during host infection was observed to be crucial for the modulation of the different survival mechanisms adopted by the bacteria. These findings reveal that the bacteria can combine diverse and complex strategies in an efficient manner to survive and persist in the environment.
PB Elsevier
SN 0740-0020
YR 2024
FD 2024-02
LK http://hdl.handle.net/10259/9284
UL http://hdl.handle.net/10259/9284
LA eng
NO This work was supported by “La Caixa” Foundation and Caja Burgos Foundation, under agreement LCF/PR/PR18/51130007. Ortega-Sanz I received a predoctoral grant from the Junta of Castile and León, cofinanced by the Ministry of Education of the Government of Castile and León and the European Social Fund.
DS Repositorio Institucional de la Universidad de Burgos
RD 19-jun-2024