RT info:eu-repo/semantics/article
T1 Phenolic Composition, Antiradical, Antimicrobial, and Anti-Inflammatory Activities of Propolis Extracts from North East Spain
A1 Osés Gómez, Sandra María
A1 Fernández Muiño, Miguel Ángel
A1 Rodríguez-Fernández, Andrea
A1 Sancho Ortiz, María Teresa
A1 Lázaro, Regina
A1 Bayarri, Susana
K1 Anti-inflammatory activity
K1 Antimicrobial activity
K1 Antiradical activity
K1 Chemical composition
K1 Propolis
K1 Alimentos-Análisis
K1 Food-Analysis
K1 Alimentos-Composición
K1 Food-Composition
AB Antioxidant-related parameters and anti-inflammatory and antimicrobial activities against Listeria monocytogenes were assessed in eight North East Spain poplar propolis samples. Propolis extracts (PEs) were obtained using 70% ethanol (PEE) and methanol (PME). Yield and total phenol compounds were higher in PEE. Phenolic acids were analyzed by a high-performance liquid chromatograph–diode array detector. Caffeic and ferulic acids were quantified in all PEE and PME. All samples contained p-coumaric acid (quantified in 6 PEE and in 3 PME). Ascorbic acid was detected in all propolis, but mainly quantified in PME (≤0.37 mg/g PE). Biological properties were tested on PEE. As for antiradical activities, trolox equivalent antioxidant capacity (TEAC) [against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)•+], ranged between 578 and 4620 µmol trolox/g, 2,2-diphenyl-1-picrylhydrazyl (DPPH) (against DPPH free radical), between 0.049 and 0.094 mg/mL, antioxidant activity against hydroxyl (•OH) radical (AOA), between 0.04 and 11.01 mmol uric acid/g, and oxygen radical absorbance capacity (ORAC) against peroxyl (ROO•) radical between 122 and 3282 µmol trolox/g. Results of TEAC, AOA, and ORAC were significantly correlated. IC50 anti-inflammatory activity ranged from 1.08 to 6.19 mg/mL. Propolis showed higher inhibitory activity against L. monocytogenes CECT934 and L. monocytogenes CP101 by agar well diffusion (P < .05) (10.5 and 10.2 mm, respectively) than against L. monocytogenes CP102 (7.0 mm). Data of this research show that North East Spain propolis may be of interest for pharmaceutical and food industry use.
PB Mary Ann Liebert
SN 1096-620X
YR 2024
FD 2024-06
LK http://hdl.handle.net/10259/9483
UL http://hdl.handle.net/10259/9483
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 30-jul-2024