RT info:eu-repo/semantics/article
T1 Low Toxicological Impact of Commercial Pristine Multi-Walled Carbon Nanotubes on the Yeast Saccharomyces cerevisiae
A1 Martel Martín, Sonia
A1 Barros García, Rocío
A1 Domi, Brixhilda
A1 Rumbo Lorenzo, Carlos
A1 Poddighe, Matteo
A1 Aparicio Martínez, Santiago
A1 Suarez Diez, Maria
A1 Tamayo Ramos, Juan Antonio
K1 Carbon nanotubes
K1 MWCNTs
K1 Saccharomyces cerevisiae
K1 Toxicity
K1 Oxidative stress
K1 Differential expression
K1 Transcriptomics
K1 Química física
K1 Chemistry, Physical and theoretical
AB Carbon nanotubes (CNTs) have attracted the attention of academy and industry due to their potential applications, being currently produced and commercialized at a mass scale, but their possible impact on different biological systems remains unclear. In the present work, an assessment to understand the toxicity of commercial pristine multi-walled carbon nanotubes (MWCNTs) on the unicellular fungal model Saccharomyces cerevisiae is presented. Firstly, the nanomaterial was physico-chemically characterized, to obtain insights concerning its morphological features and elemental composition. Afterwards, a toxicology assessment was carried out, where it could be observed that cell proliferation was negatively affected only in the presence of 800 mg L−1 for 24 h, while oxidative stress was induced at a lower concentration (160 mg L−1) after a short exposure period (2 h). Finally, to identify possible toxicity pathways induced by the selected MWCNTs, the transcriptome of S. cerevisiae exposed to 160 and 800 mg L−1, for two hours, was studied. In contrast to a previous study, reporting massive transcriptional changes when yeast cells were exposed to graphene nanoplatelets in the same exposure conditions, only a small number of genes (130) showed significant transcriptional changes in the presence of MWCNTs, in the higher concentration tested (800 mg L−1), and most of them were found to be downregulated, indicating a limited biological response of the yeast cells exposed to the selected pristine commercial CNTs.
PB MDPI
SN 2079-4991
YR 2021
FD 2021-09-01
LK http://hdl.handle.net/10259/9485
UL http://hdl.handle.net/10259/9485
LA eng
NO This work was supported by the European Union’s H2020 research and innovation programme under the grant agreements N° 691095 (NANOGENTOOLS), N° 952379 (SURFBIO), and N° 953152 (DIAGONAL); Junta de Castilla y Leon-FEDER under grant N° BU058P20 (NANOCOMP).
DS Repositorio Institucional de la Universidad de Burgos
RD 24-nov-2024