RT info:eu-repo/semantics/article
T1 Comparative toxicological analysis of two pristine carbon nanomaterials (graphene oxide and aminated graphene oxide) and their corresponding degraded forms using human in vitro models
A1 Parra de la Parra, Sandra de la
A1 Fernández Pampín, Natalia
A1 Garroni, Sebastiano
A1 Poddighe, Matteo
A1 Fuente Vivas, Dalia de la
A1 Barros García, Rocío
A1 Martel Martín, Sonia
A1 Aparicio Martínez, Santiago
A1 Rumbo Lorenzo, Carlos
A1 Tamayo Ramos, Juan Antonio
K1 A549 cells
K1 HT29 cells
K1 3D RhE model
K1 Physical degradation
K1 Química analítica
K1 Chemistry, Analytic
K1 Química física
K1 Chemistry, Physical and theoretical
AB Despite the wide application of graphene-based materials, the information of the toxicity associated to some specific derivatives such as aminated graphene oxide is scarce. Likewise, most of these studies analyse the pristine materials, while the available data regarding the harmful effects of degraded forms is very limited. In this work, the toxicity of graphene oxide (GO), aminated graphene oxide (GO-NH2), and their respective degraded forms (dGO and dGO-NH2) obtained after being submitted to high-intensity sonication was evaluated applying in vitro assays in different models of human exposure. Viability and ROS assays were performed on A549 and HT29 cells, while their skin irritation potential was tested on a reconstructed human epidermis model. The obtained results showed that GO-NH2 and dGO-NH2 substantially decrease cell viability in the lung and gastrointestinal models, being this reduction slightly higher in the cells exposed to the degraded forms. In contrast, this parameter was not affected by GO and dGO which, conversely, showed the ability to induce higher levels of ROS than the pristine and degraded aminated forms. Furthermore, none of the materials is skin irritant. Altogether, these results provide new insights about the potential harmful effects of the selected graphene-based nanomaterials in comparison with their degraded counterparts.
PB Elsevier
SN 0300-483X
YR 2024
FD 2024-05
LK http://hdl.handle.net/10259/9500
UL http://hdl.handle.net/10259/9500
LA eng
NO This work was supported by the Junta de Castilla y Leon-FEDER grant Nº  BU058P20  (NANOCOMP), and by the European Union’s H2020 research and innovation programme, under the grant agreement No  953152  (DIAGONAL). The scholarship of Matteo Poddighe received financial support within the activities of the PhD program in Chemical and Technological Sciences. We thank Graphenea for kindly providing us with the pristine GO and GO-NH2 nanomaterials.
DS Repositorio Institucional de la Universidad de Burgos
RD 27-jul-2024