RT info:eu-repo/semantics/article
T1 The Chemical Environment at Maturation Stage in Pinus spp. Somatic Embryogenesis: Implications in the Polyamine Profile of Somatic Embryos and Morphological Characteristics of the Developed Plantlets
A1 Nascimento, Antonia Maiara Marques do
A1 Polesi, Luiza Giacomolli
A1 Back, Franklin Panato
A1 Steiner, Neusa
A1 Guerra, Miguel Pedro
A1 Castander-Olarieta, Ander
A1 Moncaleán, Paloma
A1 Montalbán, Itziar Aurora
K1 Pinus halepensis
K1 Pinus radiata
K1 Sugars
K1 Amino acids
K1 Osmolality
K1 Biotecnología agraria
K1 Agricultural biotechnology
K1 Química
K1 Chemistry
AB Changes in the chemical environment at the maturation stage in Pinus spp. somatic embryogenesis will be a determinant factor in the conversion of somatic embryos to plantlets. Furthermore, the study of biochemical and morphological aspects of the somatic embryos could enable the improvement of somatic embryogenesis in Pinus spp. In the present work, the influence of different amino acid combinations, carbohydrate sources, and concentrations at the maturation stage of Pinus radiata D. Don and Pinus halepensis Mill. was analyzed. In P. radiata, the maturation medium supplemented with 175 mM of sucrose and an increase in the amino acid mixture (1,100 mgL–1 of L-glutamine, 1,050 mgL–1 of L-asparagine, 350 mgL–1 of L-arginine, and 35 mgL–1 of L-proline) promoted bigger embryos, with a larger stem diameter and an increase in the number of roots in the germinated somatic embryos, improving the acclimatization success of this species. In P. halepensis, the maturation medium supplemented with 175 mM of maltose improved the germination of somatic embryos. The increase in the amount of amino acids in the maturation medium increased the levels of putrescine in the germinated somatic embryos of P. halepensis. We detected significant differences in the amounts of polyamines between somatic plantlets of P. radiata and P. halepensis; putrescine was less abundant in both species. For the first time, in P. radiata and P. halepensis somatic embryogenesis, we detected the presence of cadaverine, and its concentration changed according to the species.
PB Frontiers
YR 2021
FD 2021-11
LK http://hdl.handle.net/10259/9788
UL http://hdl.handle.net/10259/9788
LA eng
NO This research was funded by the MINECO (Spanish Government) project (AGL2016-76143-C4-3R), CYTED (P117RT0522), and MINECO (BES-2017-081249, “Ayudas para contratos predoctorales para la formación de doctores”). MULTIFOREVER [Project MULTIFOREVER is supported under the umbrella of ERA-NET co-founded through Forest Value via ANR (FR), FNR (DE), MINCyT (AR), MINECO-AEI (ES), MMM (FI), and VINNOVA (SE)]. Forest value has received funding from the European Union’s Horizon 2020 research and innovation programmed under agreement No. 773324
DS Repositorio Institucional de la Universidad de Burgos
RD 22-dic-2024