RT info:eu-repo/semantics/article
T1 Pinus spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets
A1 Nascimento, Antonia Maiara Marques do
A1 Barroso, Priscila Alves
A1 Nascimento, Naysa Flavia Ferreira do
A1 Goicoa, Tomás
A1 Ugarte, María Dolores
A1 Montalbán, Itziar Aurora
A1 Moncaleán, Paloma
K1 Abiotic stress
K1 Embryonal masses
K1 Pinus halepensis
K1 Pinus radiata
K1 Somatic embryogenesis
K1 Somatic plantlets
K1 Tolerance
K1 Biotecnología agraria
K1 Agricultural biotechnology
K1 Química
K1 Chemistry
AB Climatic variations in the current environmental scenario require plants with tolerance to sudden changes in temperature and a decrease in water availability. Accordingly, this tolerance will enable successful plantations and the maintenance of natural and planted forests. Consequently, in the last two decades, drought tolerance and high temperatures in conifers have been an important target for morphological, physiological, and epigenetic studies. Based on this, our research team has optimized different stages of somatic embryogenesis (SE) in Pinus spp. improving the success of the process. Through this method, we can obtain a large amount of clonal material and then analyze the somatic plants under different conditions ex vitro. The analysis of the morphological and physiological parameters in somatic embryos (ses) and plants with different tolerances to abiotic stress can provide us with valuable information about the mechanisms used by plants to survive under adverse environmental conditions. Thus, the objective of this work was to evaluate the influence of high temperatures (23, 40, 50, and 60 °C, after 12 weeks, 90, 30, 5 min, respectively) on the morphology of somatic embryos obtained from Pinus radiata D.Don (Radiata pine) and Pinus halepensis Mill. (Aleppo pine). In addition, we carried out a physiological evaluation of the somatic plants of P. radiata submitted to heat and water stress in a greenhouse. We observed that the number of somatic embryos was not affected by maturation temperatures in both species. Likewise, P. radiata plants obtained from these somatic embryos survived drought and heat stress in the greenhouse. In addition, plants originating from embryonal masses (EMs) subjected to high maturation temperature (40 and 60 °C) had a significant increase in gs and E. Therefore, it is possible to modulate the characteristics of somatic plants produced by the manipulation of environmental conditions during the process of SE.
PB MDPI
YR 2020
FD 2020-11
LK http://hdl.handle.net/10259/9789
UL http://hdl.handle.net/10259/9789
LA eng
NO This research was funded by MINECO (Spanish Government) project (AGL2016-76143-C4-3R), CYTED (P117RT0522), and MINECO (BES-2017-081249, “Ayudas para contratos predoctorales para la formación de doctores”). MULTIFOREVER (Project MULTIFOREVER) is supported under the umbrella of ERA-NET cofund Forest Value by ANR (FR), FNR (DE), MINCyT (AR), MINECO-AEI (ES), MMM (FI), and VINNOVA (SE). Forest value has received funding from the European Union’s Horizon 2020 research and innovation programmed under agreement No 773324.
DS Repositorio Institucional de la Universidad de Burgos
RD 22-dic-2024