RT info:eu-repo/semantics/article
T1 Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia
A1 Merchán Carrillo, Ana
A1 Pérez Fernández, Cristian
A1 López, María J.
A1 Moreno Casco, José Joaquín
A1 Moreno-Montoya, Margarita
A1 Sánchez Santed, Fernando
A1 Flores, Pilar
K1 Compulsivity
K1 Schedule-induced polydipsia
K1 Gut microbiota
K1 Chronic tryptophan depletion
K1 PCR-DGGE
K1 Brain-derived neurotrophic factor
K1 Triptófano
K1 Tryptophan
K1 Bacterias -- Metabolismo
K1 Microbial metabolism
K1 Psicología
K1 Psychology
AB Rationale: Compulsive behaviour, present in different psychiatric disorders such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of serotonin (5-hydroxytryptamine, 5-HT). The gut microbiota regulates tryptophan (TRP) metabolism and may affect global 5-H synthesis in the enteric and central nervous systems, suggesting a possible involvement of gut microbiota in compulsive spectrum disorders.Objectives: The present study investigated whether chronic TRP depletion by diet alters the faecal bacterial community profiles of compulsive versus non-compulsive rats in schedule-induced polydipsia (SIP). Peripheral plasma 5-HT and brain-derived neurotrophic factor (BDNF) levels were evaluated.Methods: Wistar rats were selected as High Drinkers (HD) or Low Drinkers (LD) according to their SIP behaviour and were fed for 14 days with either a TRP-free diet (T-) or a TRP-supplemented diet (T+). The faecal bacterial community structure was investigated with 16S rRNA gene-targeted denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis.Results: Compulsive HD rats showed a lower bacterial diversity than LD rats, irrespectively of the diet. The TRP-depleted HD rats, the only group increasing compulsive licking in SIP, showed a reduction of bacterial evenness and a highly functionally organized community compared with the other groups, indicating that this bacterial community is more fragile to external changes due to the dominance of a low number of species. The chronic TRP depletion by diet effectively reduced peripheral plasma 5-HT levels in both HD and LD rats, while plasma BDNF levels were not altered.Conclusions: These results highlight the possible implication of reduced microbial diversity in compulsive behaviour and the involvement of the serotonergic system in modulating the gut brain-axis in compulsive spectrum disorders.
PB Elsevier
SN 0031-9384
YR 2021
FD 2021-05
LK http://hdl.handle.net/10259/9987
UL http://hdl.handle.net/10259/9987
LA eng
NO This study was funded by grants from the Ministerio de Ciencia, Innovación y Universidades (Spanish Government) and Fondo Europeo de Desarrollo Regional, PSI2017–86,847-C2–1-R and PGC2018–099,117-B-C21, and by University of Almería (PPUENTE2020/003), with the help of the Post-doctoral fellowship “Contrato Puente” at University of Almería.
DS Repositorio Institucional de la Universidad de Burgos
RD 30-ene-2025