RT info:eu-repo/semantics/article T1 l-(−)-Malic Acid Production bySaccharomycesspp. during the Alcoholic Fermentation of Wine (1) A1 Yeramian Hakim, Nadine A1 Chaya, C. A1 Suárez Lepe, J. A. K1 Wine acidity K1 Biological acidification K1 Malic acid K1 Alcoholic fermentation K1 Saccharomyces cerevisiae K1 Precursors K1 Physicochemical variables K1 Microbiología alimentaria K1 Food-Microbiology K1 Vinificación K1 Wine and wine making AB In an attempt to increase the acidity of wine by biological means, malate-producing yeasts were selected from a collection of 282 strains isolated in different parts of Spain. Only 4% of these strains (all of which belonged to Saccharomyces cerevisiae) produced l-(−)-malic acid in the range of 0.5−1 g/L. This was formed between days 2 and 6 of alcoholic fermentation, reaching a maximum on days 3 and 4; the concentration remained stable from day 7. Malic acid production was favored by temperatures in the 18−25 °C range and by musts with a high pH and low concentrations of sugar, initial malic acid, and yeast-assimilable nitrogen. Oxaloacetic acid, a precursor of malic acid, had no influence on malate production. The precursors pyruvic and fumaric acid did, however, have a significant effect on the production of this acid in some strains. No direct relation between pyruvate and malate metabolism was observed. PB American Chemical Society SN 0021-8561 YR 2007 FD 2007-01 LK http://hdl.handle.net/10259/9996 UL http://hdl.handle.net/10259/9996 LA eng DS Repositorio Institucional de la Universidad de Burgos RD 22-feb-2025