The bioavailability of phenolics contained in a powdered red wine pomace seasoning (RWPS)
and its beneficial effects after acute and short-term supplementation were investigated. First,
a single oral-dose of RWPS (300 mg/kg body weight) was administered to healthyWistar rats
(n = 6) and several phenolic acids were determined post-RWPS intake in plasma (0, 2 and
4 h) and urine (−3 to 0, 0–3 and 3–6 h). Higher prevention of lipid peroxidation (plasma and
urinary F2-isoprostanes) and improved nitric oxide bioavailability were observed in samples
collected at 4 h and between 3 and 6 h (p < 0.05), suggesting an important antioxidant role
of the metabolites generated by the action of colonic microbiota. In contrast, following 4
weeks of daily RWPS consumption, no significant differences were detected between supplemented
and control groups for any of the biomarkers assessed, which indicates that acute
beneficial effects of RWPS intake might be only transient under normal physiological conditions
for this rat model.
