Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes

Díaz de Greñu Puertas, Borja; García Calvo, José; Cuevas Vicario, José Vicente; García Herbosa, Gabriel; García Ruiz, Begoña; Busto Vázquez, Natalia; Ibeas Cortes, Saturnino; Torroba Pérez, Tomás; Torroba, Blanca; Herrera, Antonio; Pons, Sebastian

dc.contributor.author	Díaz de Greñu Puertas, Borja
dc.contributor.author	García Calvo, José
dc.contributor.author	Cuevas Vicario, José Vicente
dc.contributor.author	García Herbosa, Gabriel
dc.contributor.author	García Ruiz, Begoña
dc.contributor.author	Busto Vázquez, Natalia
dc.contributor.author	Ibeas Cortes, Saturnino
dc.contributor.author	Torroba Pérez, Tomás
dc.contributor.author	Torroba, Blanca
dc.contributor.author	Herrera, Antonio
dc.contributor.author	Pons, Sebastian
dc.date.accessioned	2017-03-14T11:36:48Z
dc.date.available	2017-03-14T11:36:48Z
dc.date.issued	2015-07
dc.identifier.uri	http://hdl.handle.net/10259/4361
dc.description.abstract	Selected new fluorogenic probes that interact in different ways with Hg2+ and MeHg+ have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg2+ and MeHg+ has been achieved by in vitro approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg2+, MeHg+ and the fluorogenic probes. In particular, MeHg+ was selectively detected in cell nuclei by probe JG45.	en
dc.description.sponsorship	the Ministerio de Econom´ıa y Competitividad, Spain (Projects CTQ2012- 31611 and CTQ2014-58812-C2-2-R), Junta de Castilla y Le´on, Consejer´ıa de Educaci´on y Cultura y Fondo Social Europeo (Project BU246A12-1), Obra Social “la Caixa” OSLC-2012-007 and the European Commission, Seventh Framework Programme (Project SNIFFER FP7-SEC-2012-312411).	en
dc.format.mimetype	application/pdf
dc.language.iso	eng	es
dc.publisher	Royal Society of Chemistry	en
dc.relation.ispartof	Chemical Science. 2015, V. 6, n. 7, p. 3757-3764	en
dc.rights	Attribution 3.0 Unported
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/
dc.subject.other	Chemistry, Organic	en
dc.subject.other	Química orgánica	es
dc.title	Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes	en
dc.type	info:eu-repo/semantics/article
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.relation.publisherversion	http://dx.doi.org/10.1039/C5SC00718F
dc.relation.projectID	info:eu-repo/grantAgreement/EC/FP7/312411
dc.type.hasVersion	info:eu-repo/semantics/publishedVersion	en

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