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dc.contributor.authorAlvarado Gámez, Ana Lorena
dc.contributor.authorAlonso Lomillo, Mª Asunción 
dc.contributor.authorDomínguez Renedo, Olga 
dc.contributor.authorArcos Martínez, Julia 
dc.date.accessioned2017-03-20T09:29:11Z
dc.date.available2017-03-20T09:29:11Z
dc.date.issued2014-02
dc.identifier.urihttp://hdl.handle.net/10259/4381
dc.description.abstractA chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap wateren
dc.description.sponsorshipResearch Vicerrectory of Costa Rica University (Project 804-B0-058) and Spanish Ministry of Science and Innovation (TEC-2009/12029). This work was supported by the Spanish Ministry of Science and Innovation (MICINN) 410 and the European Regional Development Fund (FEDER) (INNPACTO SERIBIO 2011-411 2014) and TEC2009-12029, as well as through Junta de Castilla y León (BU212A12-2).en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherMDPIen
dc.relation.ispartofSensors, 2014, V. 14, n. 2, p. 3756-3767en
dc.rightsAttribution 3.0 Unported
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectalkaline phosphataseen
dc.subjectbiosensoren
dc.subjectgold nanoparticlesen
dc.subject4-nitrophenyl phosphateen
dc.subjectscreen printed electrodeen
dc.subjectvanadiumen
dc.subjectwater analysisen
dc.subject.otherChemistry, Analyticen
dc.subject.otherQuímica analíticaes
dc.titleA Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determinationen
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttp://dx.doi.org/10.3390/s140203756
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionen


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