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dc.contributor.authorAriza Miguel, Jaime
dc.contributor.authorFernández Natal, Isabel
dc.contributor.authorSoriano, F.
dc.contributor.authorHernández Pérez, Marta 
dc.contributor.authorStessl, Beatrix
dc.contributor.authorRodríguez Lázaro, David 
dc.date.accessioned2017-04-06T11:48:16Z
dc.date.available2017-04-06T11:48:16Z
dc.date.issued2015
dc.identifier.urihttp://hdl.handle.net/10259/4410
dc.description.abstractWe investigated the pathogenicity, invasiveness, and genetic relatedness of 17 clinical Listeria monocytogenes stains isolated over a period of nine years (2006–2014). All isolates were phenotypically characterised and growth patterns were determined. The antimicrobial susceptibility of L. monocytogenes isolates was determined in E-tests. Invasion assays were performed with epithelial HeLa cells. Finally, L. monocytogenes isolates were subtyped by PFGE and MLST. All isolates had similar phenotypic characteristics (β-haemolysis and lecithinase activity), and three types of growth curve were observed. Bacterial recovery rates after invasion assays ranged from 0.09% to 7.26% (1.62 ± 0.46). MLST identified 11 sequence types (STs), and 14 PFGE profiles were obtained, indicating a high degree of genetic diversity. Genetic studies unequivocally revealed the occurrence of one outbreak of listeriosis in humans that had not previously been reported. This outbreak occurred in October 2009 and affected three patients from neighbouring towns. In conclusion, the molecular epidemiological analysis clearly revealed a cluster (three human cases, all ST1) of not previously reported listeriosis cases in northwestern Spain. Our findings indicate that molecular subtyping, in combination with epidemiological case analysis, is essential and should be implemented in routine diagnosis, to improve the tracing of the sources of outbreaks.en
dc.description.sponsorshipGerencia Regional de Salud, Junta de Castilla y Le´on, Spain, research project GRS 698/A/2011 and the EU 7th Framework Programme through the PROMISE project (Project no. 265877).en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherHindawi Publishing Corporationen
dc.relation.ispartofBioMed Research International. 2015, V. 2015, Article ID 191409, 10 pagesen
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.otherMicrobiologyen
dc.subject.otherMicrobiologíaes
dc.titleMolecular Epidemiology of Invasive Listeriosis due to Listeria monocytogenes in a Spanish Hospital over a Nine-Year Study Period, 2006–2014en
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttp://dx.doi.org/10.1155/2015/191409
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionen


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