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dc.contributor.authorGarcía Calvo, José 
dc.contributor.authorIbeas Cortes, Saturnino 
dc.contributor.authorAntón García, Eva Clara 
dc.contributor.authorTorroba Pérez, Tomás 
dc.contributor.authorGonzález Aguilar, Gerardo .
dc.contributor.authorAntunes, Wilson .
dc.contributor.authorGonzález Lavado, Eloísa .
dc.contributor.authorFanarraga, Mónica L. .
dc.date.accessioned2018-04-10T11:08:35Z
dc.date.available2018-04-10T11:08:35Z
dc.date.issued2017-08
dc.identifier.issn2191-1363
dc.identifier.urihttp://hdl.handle.net/10259/4776
dc.description.abstractWe report the development of new chemical probes for cereulide, a toxic metabolite produced by specific strains of Bacillus cereus, through displacement of potassium cations from a preformed specific complex and a subsequent change in the fluorescence emission. For this purpose, we designed fluorescent probes for potassium cations that were suitable for displacement assays with cereulide from organic extracts. The fluorescence detection of natural cereulide in rice samples was achieved by using synthetic cereulide as a reference and a potassium fluorescent reporter, and this was found to be useful as a portable and fast method for the in situ detection of cereulide in food extracts. To study the fate of cereulide in live cells, we designed a procedure that was suitable for live‐cell microscopy imaging of HeLa cells by comparing the cellular location of the potassium fluorogenic probe, which stained intracellular endolysosomes, in the absence and presence of cereulide; we concluded that in the presence of cereulide, the fluorescence of the probe was decreased because of complexation of the potassium ions by cereulide.en
dc.description.sponsorshipMinisterio de Econom&a y Competitividad, Spain (Projects CTQ2015-71353-R and AES-PI16/000496), Junta de Castilla y Lejn, Consejer&a de Educaci jn y Cultura y Fondo Social Europeo (Project BU232U13), and the European Commission, Seventh Framework Programme (Project SNIFFER FP7-SEC-2012–312411)en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherWiley-VCH Verlagen
dc.relation.ispartofChemistry Open. 2017, V. 6, n. 4, p. 562-570en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.otherChemistryen
dc.subject.otherQuímicaes
dc.titlePotassium‐ion‐selective fluorescent sensors to detect cereulide, the emetic toxin of B. cereus, in food samples and HeLa cellsen
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1002/open.201700057
dc.identifier.doi10.1002/open.201700057
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/312411
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CTQ2015-71353-R
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/AES-PI16/000496
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU232U13
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionen


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