Mostra i principali dati dell'item

dc.contributor.authorCunha Silva, Hugo 
dc.contributor.authorArcos Martínez, Julia 
dc.date.accessioned2018-07-03T10:04:19Z
dc.date.issued2018-10
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/10259/4833
dc.description.abstractLactate concentration is studied as an indicator of physical performance in sports activities, and is also analyzed in health care applications, as well as in the food and cosmetic industries. This organic acid is routinely determined in different concentration ranges, depending on the type of samples for analysis. This paper describes the development of a screen-printed lactate oxidase (LOx) based biosensor to determine lactate in broad concentration range. The Cu-MOF (copper metallic framework) crosslinking of 0.25U LOx in a chitosan layer, allows to determine the enzymatic product generated on a platinum modified working electrode, at 0.15 V (vs SPE Ag/AgCl). The biosensor responds linearly in two different concentration ranges: a first catalysis range of 14.65 µA mM−1, from 0.75 µM to 1 mM, followed by a saturation zone from 1 to 4 mM, after which a substrate enzymatic inhibition of 0.207 µA mM−1, is observed up to 50 mM. These two ranges of analysis would allow the biosensor to be used for the determination of lactate in different types of samples, with low and high content of lactate. The method reproducibility was kept below 7% and a limit of detection of 0.75 µM was obtained. The device was successfully used in the determination of lactate in sweat and saliva, as a low cost noninvasive analysis, and also in wine samples.en
dc.description.sponsorshipSpanish Ministry of Science and Innovation (MICINN) (TEC20013-40561-P), Ministry of Economy and Competitiveness (MINECO) (RTC-2015-4077-2) and the European Regional Development Fund (FEDER)en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieren
dc.relation.ispartofTalanta. 2018, V. 188, p. 779-787en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectLactate oxidaseen
dc.subjectDual range biosensoren
dc.subjectNoninvasive analysisen
dc.subjectSweaten
dc.subjectSalivaen
dc.subjectWineen
dc.subject.otherQuímica analíticaes
dc.subject.otherChemistry, Analyticen
dc.titleDual range lactate oxidase-based screen printed amperometric biosensor for analysis of lactate in diversified samplesen
dc.typeinfo:eu-repo/semantics/article
dc.date.embargo2020-10
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1016/j.talanta.2018.06.054
dc.identifier.doi10.1016/j.talanta.2018.06.054
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/RTC-2015-4077-2
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN/TEC20013-40561-P
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


Files in questo item

Thumbnail

Questo item appare nelle seguenti collezioni

Mostra i principali dati dell'item