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dc.contributor.authorBusto Vázquez, Natalia 
dc.contributor.authorCalvo Gredilla, Patricia 
dc.contributor.authorSantolaya, Javier
dc.contributor.authorLeal Villalba, José María 
dc.contributor.authorGuédin, Aurore .
dc.contributor.authorBarone, Giampaolo
dc.contributor.authorTorroba Pérez, Tomás 
dc.contributor.authorMergny, Jean-Louis
dc.contributor.authorGarcía Ruiz, Begoña 
dc.date.accessioned2018-08-24T11:28:04Z
dc.date.available2019-08-06T02:45:07Z
dc.date.issued2018-08
dc.identifier.issn0947-6539
dc.identifier.urihttp://hdl.handle.net/10259/4882
dc.description.abstractA new fluorescent, non‐cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G‐quadruplexes with scarce or no affinity towards single‐ or double‐stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G‐quadruplex structures in solution by use of PDI2B and streptavidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G‐quadruplex structures from solution, even in the presence of an excess of single‐stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2‐ over 3‐tetrad quadruplexes, which constitutes a novel type of behavior.en
dc.description.sponsorship“la Caixa” Foundation (LCF/PR/PR12/ 11070003), MINECO (CTQ2014-58812-C2-2-R and CTQ2015- 71353-R, FEDER Funds), and Junta de Castilla y Lejn, Consejer &a de Educacijn y Cultura y Fondo Social Europeo (Project BU051U16), Spain, is gratefully acknowledged. N.B. is grateful to Dr. Oscar Mendoza of the ARNA Laboratory for useful discussion and also to the financial support of the Jos8 Castillejo Program by the Spanish Ministry of Education, Culture and Sports (JC2015-00403). J.L.M. acknowledges support from Conseil Regional d’Aquitaine, Agence Nationale de la Recherche (ANR Quarpdiem) and the Symbit program [CZ.02.1.01/0.0/0.0/ 15_003/0000477] financed by the ERDF.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherWiley-VCH Verlagen
dc.relation.ispartofChemistry-a european journal. 2018, V. 24, n. 44, p. 11292-11296en
dc.subject.otherQuímicaes
dc.subject.otherChemistryen
dc.titleFishing for G-Quadruplexes in solution with a perylene diimide derivative labeled with biotinsen
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1002/chem.201802365
dc.identifier.doi10.1002/chem.201802365
dc.relation.projectIDinfo:eu-repo/grantAgreement/FundaciónLaCaixa/LCF-PR-PR12-11070003
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CTQ2014-58812-C2-2-R
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CTQ2015-71353-R
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU051U16
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


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