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dc.contributor.authorBenito Román, Oscar 
dc.contributor.authorSanz Díez, Mª Teresa 
dc.contributor.authorIllera Gigante, Alba Ester 
dc.contributor.authorMelgosa Gómez, Rodrigo 
dc.contributor.authorBenito Moreno, José Manuel 
dc.contributor.authorBeltrán Calvo, Sagrario 
dc.date.accessioned2019-01-08T12:10:08Z
dc.date.issued2019-03
dc.identifier.issn0896-8446
dc.identifier.urihttp://hdl.handle.net/10259/5045
dc.description.abstractIn the present work the effect of High Pressure Carbon Dioxide (HPCD) on the activity and structure of a commercial pectin methylesterase (PME) was studied and the results were compared to the conventional thermal inactivation technology. The use of supercritical CO2 (pressure 6–18 MPa, temperature 40–55 °C and time up to 75 min) increased dramatically the PME inactivation rate. The experimental data fitted the first order model and the inactivation kinetic study of PME was completed with the calculations of the activation energy and volume of activation. HPCD treatment induced important changes in the tertiary structure of the enzyme, as revealed the intrinsic fluorescence, KI quenching and ANS binding analyses. They showed that HPCD treatment rearranged the structure of the enzyme. These changes induced by HPCD were significantly different from those induced by mild thermal treatment. Finally, the structural changes detected correlated with the enzyme activity losses observed.en
dc.description.sponsorshipSpanish Ministry of Economy and Competitiveness for financial support of project CTQ2015-64396-R and A.E. Illera’s contract. To Junta de Castilla y León and ERDF for financial support of project BU055U16 and O. Benito-Román’s Post-doctoral contract. To Spanish Mien
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieren
dc.relation.ispartofThe Journal of Supercritical Fluids. 2019, V. 145, p. 111-121en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPMEen
dc.subjectHPCDen
dc.subjectEnzyme inactivationen
dc.subjectCO2en
dc.subjectJuiceen
dc.subject.otherIngeniería químicaes
dc.subject.otherChemical engineeringen
dc.titlePectin methylesterase inactivation by High Pressure Carbon Dioxide (HPCD)en
dc.typeinfo:eu-repo/semantics/article
dc.date.embargo2021-04-01
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1016/j.supflu.2018.11.009
dc.identifier.doi10.1016/j.supflu.2018.11.009
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CTQ2015-64396-R
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU055U16
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


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