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dc.contributor.authorIllera Gigante, Alba Ester 
dc.contributor.authorBeltrán Calvo, Sagrario 
dc.contributor.authorSanz Díez, Mª Teresa 
dc.date.accessioned2019-11-07T12:55:04Z
dc.date.available2019-11-07T12:55:04Z
dc.date.issued2019-09
dc.identifier.urihttp://hdl.handle.net/10259/5160
dc.description.abstractPolyphenoloxidase from apple was extracted and further concentrated by cationic reversed micellar extraction. Previous to reversed micellar extraction a crude protein extract was obtained using AG2-X8 as adsorbent of phenolic compounds and the detergent Triton X-100. Forward and backward extraction conditions were optimized by using dodecyl trimethyl ammonium bromide as surfactant in the organic phase. Optimization was carried out to obtain the highest value of PPO activity recovery and the purification fold at the different experimental conditions. Under the optimum extraction conditions, PPO activity recovery was 99% and purification fold reached a value of 17, showing that reversed micellar extraction was a good technique as a first step to concentrate on a targeted enzyme. After removing some impurities by centrifuge ultrafiltration, the protein extract with PPO activity was treated by pressurized carbon dioxide and thermosonication achieving residual PPO activity values of 16 ± 3 and 9 ± 1%, respectively. Quenching experiments by iodide performed in the non-treated extract and in the treated extracts revealed conformational changes of this protein fraction reflected in the greater exposure of the fluorophore to the quencher.en
dc.description.sponsorshipSpanish Government (MINECO) and the European Regional Development Fund (ERDF) for financial support of project CTQ2015-64396-R and AEI’s contract, and to Junta de Castilla y León and ERDF for financial support of project BU301P18.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherNature Research
dc.relation.ispartofScientific Reports. 2019, V. 9, art. 13749
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherIngeniería químicaes
dc.subject.otherChemical engineeringen
dc.titleStructural changes of a protein extract from apple with polyphenoloxidase activity obtained by cationic reversed micellar extraction induced by high-pressure carbon dioxide and thermosonicationen
dc.typeinfo:eu-repo/semantics/article
dc.rights.holderAtribución 4.0 Internacional*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-019-50209-w
dc.identifier.doi10.1038/s41598-019-50209-w
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CTQ2015-64396-R
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU301P18
dc.identifier.essn2045-2322
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion


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