Mostrar registro simples

dc.contributor.authorSuarez Diez, Maria
dc.contributor.authorPorras Alfonso, Santiago 
dc.contributor.authorLaguna Teno, Félix
dc.contributor.authorSchaap, Peter J.
dc.contributor.authorTamayo Ramos, Juan Antonio 
dc.date.accessioned2020-03-02T09:19:39Z
dc.date.available2020-03-02T09:19:39Z
dc.date.issued2020-02
dc.identifier.urihttp://hdl.handle.net/10259/5225
dc.description.abstractGraphene nanomaterials have attracted a great interest during the last years for different applications, but their possible impact on different biological systems remains unclear. Here, an assessment to understand the toxicity of commercial polycarboxylate functionalized graphene nanoplatelets (GN) on the unicellular fungal model Saccharomyces cerevisiae was performed. While cell proliferation was not negatively affected even in the presence of 800 mg L−1 of the nanomaterial for 24 hours, oxidative stress was induced at a lower concentration (160 mg L−1), after short exposure periods (2 and 4 hours). No DNA damage was observed under a comet assay analysis under the studied conditions. In addition, to pinpoint the molecular mechanisms behind the early oxidative damage induced by GN and to identify possible toxicity pathways, the transcriptome of S. cerevisiae exposed to 160 and 800 mg L−1 of GN was studied. Both GN concentrations induced expression changes in a common group of genes (337), many of them related to the fungal response to reduce the nanoparticles toxicity and to maintain cell homeostasis. Also, a high number of genes were only differentially expressed in the GN800 condition (3254), indicating that high GN concentrations can induce severe changes in the physiological state of the yeast.en
dc.description.sponsorshipEuropean Union’s H2020 research and innovation programme under the Marie Skłodowska-Curie grant agreements N° 691095 and N° 734873; and Junta de Castilla y Leon-FEDER under grants N° BU079U16, and N° UBU-16-B.es
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherNature Researches
dc.relation.ispartofScientific Reports. 2020, 10, art. 3232es
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherMaterialeses
dc.subject.otherMaterialsen
dc.titleToxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplateletsen
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-020-60101-7es
dc.identifier.doi10.1038/s41598-020-60101-7
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/691095
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/734873
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU079U16
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/UBU-16-B
dc.identifier.essn2045-2322
dc.journal.titleScientific Reportses
dc.volume.number10es
dc.issue.number1es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion


Arquivos deste item

Thumbnail

Este item aparece na(s) seguinte(s) coleção(s)

Mostrar registro simples