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dc.contributor.authorArnáiz, Esther
dc.contributor.authorMoreno Mediavilla, Daniel 
dc.contributor.authorQuesada Pato, Roberto 
dc.date.accessioned2020-07-10T11:23:39Z
dc.date.available2020-07-10T11:23:39Z
dc.date.issued2014-03
dc.identifier.issn0003-2719
dc.identifier.urihttp://hdl.handle.net/10259/5371
dc.description.abstractThe extraction and determination of volatile compounds in mice urine were performed using headspace solid-phase microextraction and gas chromatography-mass spectrometry. In order to optimize the extraction conditions, experimental design was applied. A sample volume of 108 µl, a temperature of 148.6°C, and a time of 94 minutes were found to be the optimal conditions. Samples of male and female mouse urine were analyzed to determine volatile compound profiles. A total of 36 organic compounds including ketones, aldehydes, and terpenes were detected. The results revealed that compounds such as 2-isopropyl-4,5-dihydrothiazole, which is considered a male sexual pheromone, were only detected in male urine samples, whereas others like benzaldehyde were especially abundant in female mouse urine. A comparison of female samples corresponding to different stages of the estrous cycle was also performed.en
dc.description.sponsorshipEuropean Commission ([SME-2011-1] Research for SMEs, PiedPiper project, Grant agreement: 286852)es
dc.format.mimetypeapplication/pdf
dc.language.isospaes
dc.publisherTaylor & Francis Groupes
dc.relation.ispartofAnalytical Letters. 2014, V. 47, n. 5, p. 721-729es
dc.subjectExperimental designen
dc.subjectGas chromatography-mass spectrometry (GC-MS)en
dc.subjectHeadspace-solid-phase micro-extraction (HS-SPME)en
dc.subjectMouse urineen
dc.subjectVolatile organic compoundsen
dc.subject.otherQuímica orgánicaes
dc.subject.otherChemistry, Organicen
dc.titleDetermination of Volatiles in Mouse Urine by Headspace Solid Phase Microextraction and Gas Chromatography-Mass Spectrometryen
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1080/00032719.2013.853182
dc.identifier.doi10.1080/00032719.2013.853182
dc.identifier.essn1532-236X
dc.journal.titleAnalytical Letterses
dc.volume.number47es
dc.issue.number5es
dc.page.initial721es
dc.page.final729es
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion


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