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dc.contributor.authorMacii, Francesca
dc.contributor.authorPérez Arnáiz, Cristina 
dc.contributor.authorArrico, Lorenzo
dc.contributor.authorBusto Vázquez, Natalia 
dc.contributor.authorGarcía Ruiz, Begoña 
dc.contributor.authorBiver, Tarita
dc.date.accessioned2020-09-22T11:01:45Z
dc.date.available2020-09-22T11:01:45Z
dc.date.issued2020-11
dc.identifier.issn0162-0134
dc.identifier.urihttp://hdl.handle.net/10259/5475
dc.description.abstractThis work concerns an analysis of the binding mechanism of a copper phthalocyanine (Alcian Blue-tetrakis(methylpyridinium) chloride, ABTP) to natural calf thymus DNA, G-quadruplexes (G4) and synthetic RNA polynucleotides in the form of double polyriboadenylic·polyribouridylic acid (poly(A)·poly(U)) or triple strands polyriboadenylic·2polyribouridylic acid (poly(A)·2poly(U)). ABTP is a well know dye that might undergo novel applications, but its interaction with DNA is scarcely studied and we lack information on possible RNA or G4 binding. This might be related to system complexity due to the presence of supramolecular dye-dye aggregates. Despite this, we show here that apparent parameters can be calculated, which provide information on the binding mechanism. Absorbance titrations in the presence of biosubstrate excess, melting and circular dichroism experiments show that ABTP binds to both RNAs and DNA. External/groove binding is the main feature for RNAs, whereas partial intercalation is the major binging mode for DNA. ABTP externally binds to both hybrid, parallel and anti-parallel G4s but seem to show a slightly different binding mode and a preference for anti-parallel structures. The thermodynamic features of the different systems are also discussed in the frame of the enthalpy-entropy compensation phenomenon.en
dc.description.sponsorshipla Caixa” Foundation (LCF/PR/PR12/11070003), Ministerio de Ciencia, Innovación y Universidades-FEDER (RTI2018- 102040-B-100) and Junta de Castilla y León-FEDER (BU305P18)es
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofJournal of Inorganic Biochemistry. 2020, V. 212, 111199es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectGroove bindingen
dc.subjectPartial intercalationen
dc.subjectHelix conformationen
dc.subjectEnthalpy-entropy compensationen
dc.subjectAnti-parallelen
dc.subjectHybriden
dc.subject.otherQuímica inorgánicaes
dc.subject.otherChemistry, Inorganicen
dc.subject.otherBioquímicaes
dc.subject.otherBiochemistryen
dc.titleAlcian blue pyridine variant interaction with DNA and RNA polynucleotides and G-quadruplexes: changes in the binding features for different biosubstratesen
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1016/j.jinorgbio.2020.111199
dc.identifier.doi10.1016/j.jinorgbio.2020.111199
dc.relation.projectIDinfo:eu-repo/grantAgreement/FundaciónLaCaixa/LCF/PR/PR12/11070003
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN/RTI2018- 102040-B-100
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/U305P18
dc.journal.titleJournal of Inorganic Biochemistryes
dc.volume.number212es
dc.page.initial111199es
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion


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