The utilization of tungsten disulfide (WS2) nanomaterials in distinct applications is raising due to their unique physico-chemical properties, such as low friction coefficient and high strength, which highlights the necessity to study their potential toxicological effects, due to the potential increase of environmental and human exposure. The aim of this work was to analyze commercially available aqueous dispersions of monolayer tungsten disulfide (2D WS2) nanomaterials with distinct lateral size employing a portfolio of physico-chemical and toxicological evaluations. The structure and stoichiometry of monolayer tungsten disulfide (WS2-ACS-M) and nano size monolayer tungsten disulfide (WS2-ACS-N) was analyzed by Raman spectroscopy, whereas a more quantitative approach to study the nature of formed oxidized species was undertaken employing X-ray photoelectron spectroscopy. Adenocarcinomic human alveolar basal epithelial cells (A549 cells) and the ecotoxicology model Saccharomyces cerevisiae were selected as unicellular eukaryotic systems to assess the cytotoxicity of the nanomaterials. Cell viability and reactive oxygen species (ROS) determinations demonstrated different toxicity levels depending on the cellular model used. While both 2D WS2 suspensions showed very low toxicity towards the A549 cells, a comparable concentration (160 mg L−1) reduced the viability of yeast cells. The toxicity of a nano size 2D WS2 commercialized in dry form from the same provider was also assessed, showing ability to reduce yeast cells viability as well. Overall, the presented data reveal the physico-chemical properties and the potential toxicity of commercial 2D WS2 aqueous suspensions when interacting with distinct eukaryotic organisms, showing differences in function of the biological system exposed.
