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dc.contributor.authorSchindler, Torben
dc.contributor.authorGonzález, Adrián
dc.contributor.authorBoopathi, Ramachandran
dc.contributor.authorMarty Roda, Marta 
dc.contributor.authorRomero Santacreu, Lorena 
dc.contributor.authorWildes, Andew R.
dc.contributor.authorPorcar, Lionel
dc.contributor.authorMartel, Anne
dc.contributor.authorTheodorakopoulos, Nikos
dc.contributor.authorCuesta López, Santiago 
dc.contributor.authorAngelov, Dimitar
dc.contributor.authorUnruh, Tobias
dc.contributor.authorPeyrard, Michel
dc.date.accessioned2021-05-25T10:06:10Z
dc.date.available2021-05-25T10:06:10Z
dc.date.issued2018-10
dc.identifier.issn2470-0045
dc.identifier.urihttp://hdl.handle.net/10259/5784
dc.description.abstractDNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted persistence length of lp ≈ 500Å is inconsistent with recent studies on short-chain DNA that show much greater flexibility but do not probe its origin. We have performed x-ray and neutron small-angle scattering on a short DNA sequence containing a strong nucleosome positioning element and analyzed the results using a modified Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our method can detect a locally enhanced flexibility due to an AT domain.en
dc.description.sponsorshipSpanish Ministry of Economy, Industry and Competitiveness (BES-2013-065453, EEBB-I-2015-09973, FIS2012-38827). S.C.L. and UC-154 are grateful for the support of Junta de Castilla y Leon (Spain) Nanofibersafe BU079U16. D.A. acknowledges funding from the Agence Nationale de la Recherche through ANR-12-BSV5-0017-01 “Chrome” and ANR-17-CE11-0019-03 “Chrom3D” grants. N.T. acknowledges support by the project Advanced Materials and Devices (MIS 5002409, Competitiveness, Entrepreneurship and Innovation, NSRF 2014-2020) cofinanced by Greece and the European Regional Development Fund.es
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherAmerican Physical Societyes
dc.relation.ispartofPhysical Review E. 2018, V. 98, n. 4, 042417es
dc.subject.otherFísicaes
dc.subject.otherPhysicsen
dc.titleKinky DNA in solution: Small-angle-scattering study of a nucleosome positioning sequenceen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1103/PhysRevE.98.042417es
dc.identifier.doi10.1103/PhysRevE.98.042417
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/BES-2013-065453
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/EEBB-I-2015-09973
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/FIS2012-38827
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU079U16
dc.identifier.essn2470-0053
dc.journal.titlePhysical Review Ees
dc.volume.number98es
dc.issue.number4es
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones


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