dc.contributor.author | Prieto Santiago, Virginia | |
dc.contributor.author | Cavia Camarero, María del Mar | |
dc.contributor.author | Barba, Francisco J. | |
dc.contributor.author | Alonso de la Torre, Sara | |
dc.contributor.author | Carrillo Pérez, Celia | |
dc.date.accessioned | 2021-11-22T07:52:22Z | |
dc.date.available | 2021-11-22T07:52:22Z | |
dc.date.issued | 2022-01 | |
dc.identifier.issn | 0308-8146 | |
dc.identifier.uri | http://hdl.handle.net/10259/6196 | |
dc.description.abstract | Raffinose family oligosaccharides are non-digestible compounds considered as dietary prebiotics with health-related properties. Hence, it is important to develop highly specific methods for their determination. An analytical method is developed in this study for oligosaccharide identification and quantification using liquid chromatography-tandem mass spectrometry equipped with a triple quadrupole analyser operating in Multiple Reaction Monitoring mode. Raffinose, stachyose and verbascose are separated in a 10-minute run and the method is validated over a broad concentration range, showing good linearity, accuracy, precision and high sensitivity. A low-cost, short eco-friendly procedure for oligosaccharide extraction from legumes, with a high recovery rate extraction, good repeatability and reproducibility is also proposed. No plant-matrix effects were demonstrated. The method applied to the screening of 28 different legumes revealed species-related traits for oligosaccharide distribution, highlighting Pisum sativum (9.22 g/100 g) as the richest source of these prebiotics and its suitability as a functional food ingredient. | en |
dc.description.sponsorship | Prieto-Santiago is a research assistant funded by Junta de Castilla y León (Spain) (Grant No. UBU-10-B). | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Food Chemistry. 2022, V. 368, 130761 | en |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Triple quadrupole | en |
dc.subject | Multiple reaction monitoring | en |
dc.subject | Oligosaccharides | en |
dc.subject | Legumes | en |
dc.subject.other | Biología molecular | es |
dc.subject.other | Molecular biology | en |
dc.subject.other | Alimentos | es |
dc.subject.other | Food | en |
dc.title | Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer | en |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.foodchem.2021.130761 | es |
dc.identifier.doi | 10.1016/j.foodchem.2021.130761 | |
dc.relation.projectID | info:eu-repo/grantAgreement/Junta de Castilla y León//UBU-10-B | |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es |