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dc.contributor.authorArnáiz Alonso, Ana 
dc.contributor.authorGuembe García, Marta 
dc.contributor.authorDelgado-Pinar, Estefanía
dc.contributor.authorValente, Artur J. M.
dc.contributor.authorIbeas Cortes, Saturnino 
dc.contributor.authorGarcía Pérez, José Miguel 
dc.contributor.authorVallejos Calzada, Saúl 
dc.date.accessioned2022-05-30T12:37:10Z
dc.date.available2022-05-30T12:37:10Z
dc.date.issued2022-05
dc.identifier.urihttp://hdl.handle.net/10259/6685
dc.description.abstractWe have faced the preparation of fully water-soluble fluorescent peptide substrate with long-term environmental stability (in solution more than 35 weeks) and, accordingly, with stable results in the use of this probe in determining the activity of enzymes. We have achieved this goal by preparing a co-polymer of the commercial N-vinyl-2-pyrrolidone (99.5% mol) and a fluorescent substrate for trypsin activity determination having a vinylic group (0.5%). The activity of trypsin has been measured in water solutions of this polymer over time, contrasted against the activity of both the commercial substrate Z-L-Arg-7-amido-4-methylcoumarin hydrochloride and its monomeric derivative, prepared ad-hoc. Initially, the activity of the sensory polymer was 74.53 ± 1.72 nmol/min/mg of enzyme, while that of the commercial substrate was 20.44 ± 0.65 nmol/min/mg of enzyme, the former maintained stable along weeks and the latter with a deep decay to zero in three weeks. The ‘protection’ effect exerted by the polymer chain has been studied by solvation studies by UV–Vis spectroscopy, steady-state & time resolved fluorescence, thermogravimetry and isothermal titration calorimetry.en
dc.description.sponsorship"La Caixa" Foundation, under agreement LCF/PR/PR18/51130007. We also gratefully acknowledge the financial support provided by FEDER (Fondo Europeo de Desarrollo Regional) through Programa Operacional Factores de Competitividade (COMPETE), and the Spanish State Research Agency (PID2020-113264RB-I00/AEI/10.13039/501100011033). Centro de Química de Coimbra acknowledges Fundação para a Ciência e Tecnologia for financial support (Projects UIDB/00313/2020, UIDP/00313/2020). E.D.-P. thanks the “Concurso de Estímulo ao Emprego Científico” for the junior contract CEECIND/04136/2018.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherSpringer Naturees
dc.relation.ispartofScientific Reports. 2022, V. 12, 8818es
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectActuatorsen
dc.subjectSensors and biosensorsen
dc.subject.otherQuímica orgánicaes
dc.subject.otherChemistry, Organicen
dc.titleThe role of polymeric chains as a protective environment for improving the stability and efficiency of fluorogenic peptide substratesen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-022-12848-4es
dc.identifier.doi10.1038/s41598-022-12848-4
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIOes
dc.relation.projectIDinfo:eu-repo/grantAgreement/FCT//UIDB%2F00313%2F2020
dc.relation.projectIDinfo:eu-repo/grantAgreement/FCT//UIDP%2F00313%2F2020
dc.identifier.essn2045-2322
dc.journal.titleScientific Reportsen
dc.volume.number12es
dc.issue.number1es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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