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dc.contributor.authorArnáiz Alonso, Ana 
dc.contributor.authorGuirado Moreno, José Carlos 
dc.contributor.authorGuembe García, Marta 
dc.contributor.authorBarros García, Rocío 
dc.contributor.authorTamayo Ramos, Juan Antonio 
dc.contributor.authorFernández Pampín, Natalia 
dc.contributor.authorGarcía Pérez, José Miguel 
dc.contributor.authorVallejos Calzada, Saúl 
dc.date.accessioned2023-01-25T12:38:53Z
dc.date.available2023-01-25T12:38:53Z
dc.date.issued2023-03
dc.identifier.issn0925-4005
dc.identifier.urihttp://hdl.handle.net/10259/7316
dc.description.abstractThe initial stages of the pandemic caused by SARS-CoV-2 showed that early detection of the virus in a simple way is the best tool until the development of vaccines. Many different tests are invasive or need the patient to cough up or even drag a sample of mucus from the throat area. Besides, the manufacturing time has proven insufficient in pandemic conditions since they were out of stock in many countries. Here we show a new method of manufacturing virus sensors and a proof of concept with SARS-CoV-2. We found that a fluorogenic peptide substrate of the main protease of the virus (Mpro) can be covalently immobilized in a polymer, with which a cellulose-based material can be coated. These sensory labels fluoresce with a single saliva sample of a positive COVID-19 patient. The results matched with that of the antigen tests in 22 of 26 studied cases (85% success rate).en
dc.description.sponsorshipWe gratefully acknowledge the financial support provided by all funders. Author Saul Vallejos coordinates the project leading to these results, which has received funding from "La Caixa" Foundation, under agreement LCF/PR/PR18/51130007. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author Jose Miguel García received grant PID2020–113264RB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. Ana Arnaiz received funding from Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). Finally, all the authors want to thank the support provided by City Hall of Villadiego “Ayuntamiento de Villadiego” when looking for participants for the proof of concept.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofSensors and Actuators B: Chemical. 2023, V. 379, 133165en
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPeptideen
dc.subjectSubstrateen
dc.subjectFluorimetryen
dc.subjectImmobilizationen
dc.subjectCOVID-19en
dc.subjectPaper-supporteden
dc.subjectCoatingen
dc.subjectPandemicsen
dc.subject.otherQuímica orgánicaes
dc.subject.otherChemistry, Organicen
dc.subject.otherEnfermedades infecciosases
dc.subject.otherCommunicable diseasesen
dc.titleLab-on-a-chip for the easy and visual detection of SARS-CoV-2 in saliva based on sensory polymersen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1016/j.snb.2022.133165es
dc.identifier.doi10.1016/j.snb.2022.133165
dc.relation.projectIDinfo:eu-repo/grantAgreement/Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona//LCF%2FPR%2FPR18%2F51130007/es
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO/es
dc.journal.titleSensors and Actuators B: Chemicales
dc.volume.number379es
dc.page.initial133165es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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