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dc.contributor.authorParra de la Parra, Sandra de la 
dc.contributor.authorGonzález, Verónica
dc.contributor.authorSolórzano Vives, Patricia
dc.contributor.authorCuriel Alegre, Sandra 
dc.contributor.authorVelasco Arroyo, Blanca 
dc.contributor.authorRad Moradillo, Juan Carlos 
dc.contributor.authorBarros García, Rocío 
dc.contributor.authorTamayo Ramos, Juan Antonio 
dc.contributor.authorRumbo Lorenzo, Carlos 
dc.date.accessioned2023-03-06T07:36:45Z
dc.date.available2023-03-06T07:36:45Z
dc.date.issued2022-12
dc.identifier.issn0269-7491
dc.identifier.urihttp://hdl.handle.net/10259/7490
dc.description.abstractThe biological effects induced by the pollutants present in soils, together with the chemical and physical characterizations, are good indicators to provide a general overview of their quality. However, the existence of studies where the toxicity associated to soils contaminated with mixtures of pollutants applying both in vitro and in vivo models are scarce. In this work, three soils (namely, Soil 001, Soil 002 and Soil 013) polluted with different concentrations of hydrocarbons and heavy metals were evaluated using different organisms representative of human (HepG2 human cell line) and environmental exposure (the yeast Saccharomyces cerevisiae, the Gram-negative bacterium Pseudomonas putida and, for the in vivo evaluation, the annelid Enchytraeus crypticus). In vitro assays showed that the soluble fraction of the Soil 001, which presented the highest levels of heavy metals, represented a great impact in the viability of the HepG2 cells and S. cerevisiae, while organic extracts from Soils 002 and 013 caused a slight decrease in the viability of HepG2 cells. In addition, in vivo experiments showed that Soils 001 and 013 affected the survival and the reproduction of E. crypticus. Altogether, these results provide a general overview of the potential hazards associated to three specific contaminated sites in a variety of organisms, showing how different concentrations of similar pollutants affect them, and highlights the relevance of testing both organic and soluble extracts when in vitro safety assays of soils are performed.en
dc.description.sponsorshipThis work received funding from the GREENER project of the European Union’s Horizon 2020 research and innovation program (Grant Agreement No. 826312). S. Curiel-Alegre was granted with a predoctoral contract by Junta de Castilla y León and the European Social Fund (ORDEN EDU/1508/2020, de 15 de diciembre). We thank Mireya Pedrero and Andrea Martínez for their technical support. We also thank Institute of Technology Carlow and Shandong Academy of Sciences for kindly providing us with soil samples.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieren
dc.relation.ispartofEnvironmental Pollution. 2022, V. 315, 120472en
dc.rightsAtribución-NoComercial 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectHepG2 cellsen
dc.subjectSaccharomyces cerevisiaeen
dc.subjectPseudomonas putidaen
dc.subjectEnchytraeus crypticusen
dc.subjectSoil contaminationen
dc.subject.otherQuímica analíticaes
dc.subject.otherChemistry, Analyticen
dc.titleComparative toxicological assessment of three soils polluted with different levels of hydrocarbons and heavy metals using in vitro and in vivo approachesen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1016/j.envpol.2022.120472es
dc.identifier.doi10.1016/j.envpol.2022.120472
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/826312/EU/InteGRated systems for Effective ENvironmEntal Remediation/GREENER/en
dc.journal.titleEnvironmental Pollutionen
dc.volume.number315es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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