Double emulsions are ternary systems commonly used in several disciplines in areas such
as food technology, applied chemistry, chemical engineering, materials science, pharmacology and
environmental science. In several courses related to these areas, the implementation of laboratory experiment is required to strengthen the knowledge acquired by students during the theoretical lessons.
However, it is difficult to find published practical experiments in this field. This work presents a
four-hour hands-on laboratory experiment in which students can easily formulate and prepare waterin-oil-in-water double emulsions for vitamin B12 encapsulation. In this experiment, students can
analyze the effect of the osmotic pressure produced by the addition of different NaCl concentrations
in each aqueous phase, which could lead to the swelling and deswelling phenomena of the inner
aqueous droplets and, therefore, affect the encapsulation efficiency of the formulated systems. The
double emulsions are analyzed by the students in terms of size and encapsulation efficiency
