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dc.contributor.authorArnáiz Alonso, Ana 
dc.contributor.authorVallejo García, Jorge Lucas 
dc.contributor.authorVallejos Calzada, Saúl 
dc.contributor.authorDiaz, Isabel
dc.date.accessioned2023-06-21T11:57:21Z
dc.date.available2023-06-21T11:57:21Z
dc.date.issued2023-06
dc.identifier.issn2331-8325
dc.identifier.urihttp://hdl.handle.net/10259/7714
dc.descriptionProtocolo
dc.description.abstractMandelonitrile is a nitrogen-containing compound, considered an essential secondary metabolite. Chemically, it is a cyanohydrin derivative of benzaldehyde, with relevant functions in different physiological processes including defense against phytophagous arthropods. So far, procedures for detecting mandelonitrile have been effectively applied in cyanogenic plant species such as Prunus spp. Nevertheless, its presence in Arabidopsis thaliana, considered a non-cyanogenic species, has never been determined. Here, we report the development of an accurate protocol for mandelonitrile quantification in A. thaliana within the context of A. thaliana–spider mite interaction. First, mandelonitrile was isolated from Arabidopsis rosettes using methanol; then, it was derivatized by silylation to enhance detection and, finally, it was quantified using gas chromatography–mass spectrometry. The selectivity and sensitivity of this method make it possible to detect low levels of mandelonitrile (LOD 3 ppm) in a plant species considered non-cyanogenic that, therefore, will have little to no cyanogenic compounds, using a small quantity of starting material (≥ 100 mg).en
dc.description.sponsorshipThis work was supported by funds of “La Caixa” Foundation (LCF/PR18/51130007) and Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid).en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherBio-protocol LLCen
dc.relation.ispartofBio-protocol. 2023, V. 13, n. 12, e4700en
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBenzaldehyde cyanohydrinen
dc.subjectMandelonitrileen
dc.subjectHydroxynitrile lyaseen
dc.subjectArabidopsis thalianaen
dc.subjectSpider miteen
dc.subjectCyanogenic glycosidesen
dc.subjectCyanohydrinen
dc.subject.otherQuímica orgánicaes
dc.subject.otherChemistry, Organicen
dc.titleIsolation and Quantification of Mandelonitrile from Arabidopsis thaliana Using Gas Chromatography/Mass Spectrometryen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.21769/BioProtoc.4700es
dc.identifier.doi10.21769/BioProtoc.4700
dc.relation.projectIDinfo:eu-repo/grantAgreement/Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona//LCF%2FPR%2FPR18%2F51130007//Fluoración Directa de Nitrocompuestos y Sales de Heteroaril Fosfonio: Síntesis de Fluorocompuestos/FluNitroPhos/es
dc.journal.titleBIO-PROTOCOLen
dc.volume.number13es
dc.issue.number12es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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