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dc.contributor.authorVallejo García, Jorge Lucas 
dc.contributor.authorArnáiz Alonso, Ana 
dc.contributor.authorBusto Núñez, Mª Dolores 
dc.contributor.authorGarcía Pérez, José Miguel 
dc.contributor.authorVallejos Calzada, Saúl 
dc.date.accessioned2023-11-02T11:56:45Z
dc.date.available2023-11-02T11:56:45Z
dc.date.issued2023-11
dc.identifier.issn0014-3057
dc.identifier.urihttp://hdl.handle.net/10259/7922
dc.description.abstractIn this study, we report the synthesis and characterization of a highly manageable polyacrylic film material for enzyme immobilization, using β-galactosidase (β-gal) as a model enzyme. The material is based on commercially available monomers and achieves efficient immobilization of β-gal through the formation of azo linkages between amino styrene groups in the polyacrylic material and the enzyme. The immobilized enzyme demonstrates superior performance compared to free enzyme in lactose hydrolysis of UHT milk, achieving lactose concentrations below 0.1% (<1 mg/mL), indicating its potential for lactose hydrolysis in dairy products. The film-shaped material is designed for easy submersion and removal, similar to a smart card, and offers reusability, with the ability to be reused at least 10 times without loss of enzymatic activity. Characterization of the immobilized enzyme on the polymeric material was performed using various techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Protein release studies confirmed the stability of the immobilized enzyme during prolonged incubation in aqueous solution without significant enzyme leakage. Overall, the polyacrylic film material demonstrates promise as a simple and efficient approach for enzyme immobilization, with potential applications in various industries, including the food industry.en
dc.description.sponsorshipWe gratefully acknowledge the financial support provided by all funders. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author J. M. García received grant PID2020-113264RB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. A. Arnaiz received funding from Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). J. L. Vallejo-García received the grant PRE2021-09812 funded by MCIN/AEI/ 10.13039/501100011033 and by “ESF Investing in your future”. Author Saul Vallejos received grant BG22/00086 funded by Spanish Ministerio de Universidades.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofEuropean Polymer Journal. 2023, V. 200, 112495en
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectβ-galactosidaseen
dc.subjectLactose-freeen
dc.subjectLow-lactoseen
dc.subjectLactose hydrolysisen
dc.subjectEnzyme inmobilizationen
dc.subject.otherQuímica orgánicaes
dc.subject.otherChemistry, Organicen
dc.subject.otherBioquímicaes
dc.subject.otherBiochemistryen
dc.subject.otherAlimentoses
dc.subject.otherFooden
dc.titleFilm-shaped reusable smart polymer to produce lactose-free milk by simple immersionen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1016/j.eurpolymj.2023.112495es
dc.identifier.doi10.1016/j.eurpolymj.2023.112495
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO/es
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023/PRE2021-098123/ES/es
dc.relation.projectIDinfo:eu-repo/grantAgreement/MIU/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/BG22%2F00086/ES/es
dc.journal.titleEuropean Polymer Journalen
dc.volume.number200es
dc.page.initial112495es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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