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dc.contributor.authorDurán Fernández-Feijóo, Cristina
dc.contributor.authorRodríguez-Fanjul, Javier
dc.contributor.authorLópez-Abat, Miriam
dc.contributor.authorHadley, Stephanie
dc.contributor.authorCavia Saiz, Mónica 
dc.contributor.authorMuñiz Rodríguez, Pilar 
dc.contributor.authorArnaez, Juan
dc.contributor.authorFernández-Lorenzo, José Ramón
dc.contributor.authorCamprubí Camprubí, Marta
dc.date.accessioned2023-11-21T08:12:55Z
dc.date.available2023-11-21T08:12:55Z
dc.date.issued2021-09
dc.identifier.urihttp://hdl.handle.net/10259/8069
dc.description.abstractHypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidative damage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Our aim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day rat pups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated, hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia + allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH/GSSG ratio and carbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma and cerebrospinal fluid, and 8-iso-prostaglandin F2α was measured in brain tissue. Plasma 2,20–azinobis- (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that received allopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferric reducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantly reduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotection in HIE.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherMDPIes
dc.relation.ispartofAntioxidants. 2021, V. 10, n. 10, 1523es
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAllopurinolen
dc.subjectHypothermiaen
dc.subjectHypoxic-ischemic encephalopathyen
dc.subjectOxidative stressen
dc.subjectOxidative damageen
dc.subject.otherBioquímicaes
dc.subject.otherBiochemistryen
dc.subject.otherBiología moleculares
dc.subject.otherMolecular biologyen
dc.titleEffects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathyen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.3390/antiox10101523es
dc.identifier.doi10.3390/antiox10101523
dc.identifier.essn2076-3921
dc.journal.titleAntioxidantsen
dc.volume.number10es
dc.issue.number10es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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