Show simple item record

dc.contributor.authorMvila, Beaufray Gilaime
dc.contributor.authorPilar Izquierdo, María Concepción 
dc.contributor.authorBusto Núñez, Mª Dolores 
dc.contributor.authorPérez Mateos, Manuel 
dc.contributor.authorOrtega Santamaría, Natividad 
dc.date.accessioned2024-01-09T12:04:02Z
dc.date.available2024-01-09T12:04:02Z
dc.date.issued2015-09
dc.identifier.issn0022-5142
dc.identifier.urihttp://hdl.handle.net/10259/8264
dc.description.abstractBACKGROUND Most N fertilizers added to soil are not efficiently used by plants and are lost to the atmosphere or leached from the soil, causing environmental pollution and increasing cost. Barley seed encapsulation in calcium alginate gels containing free or immobilized urease to enhance plant utilization of soil N was investigated. RESULTS Urease was immobilized with soil humic acids (HA). A central composite face-centered design was applied to optimize the immobilization process, reaching an immobilization yield of 127%. Soil stability of urease was enhanced after the immobilization. Seed encapsulation with free urease (FU) and humic–urease complex (HUC) resulted in a urease activity retention in the coating layer of 46% and 24%, and in germination rates of 87% and 92%, respectively. Under pot culture conditions, the pots planted with seeds encapsulated with FU and HUC showed higher ammonium N (NH4+-N) (26% and 64%, respectively) than the control soil at 28 days after planting (DAP). Moreover, the seed encapsulation with FU and HUC increased the N uptake 83% and 97%, respectively, at 35 DAP. CONCLUSION Seed encapsulation with urease could substantially contribute to enhancing plant N nutrition in the early stages of seedling establishment.en
dc.description.sponsorshipWe thank the ‘Ministere de L’Enseignement Superieur de laRepublique du Congo’ for a fellowship awarded to BG Mvila.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherSociety of Chemical Industryen
dc.relation.ispartofJournal of the Science of Food and Agriculture. 2016, V. 96, n. 9. p. 2981-2989en
dc.subjectEnzyme immobilizationen
dc.subjectNitrogen availabilityen
dc.subjectPlant growthen
dc.subjectSeed coatingen
dc.subjectSeed encapsulationen
dc.subjectSoil ureaseen
dc.subject.otherBioquímicaes
dc.subject.otherBiochemistryen
dc.subject.otherAgriculturaes
dc.subject.otherAgricultureen
dc.titleSynthesis and characterization of a stable humic–urease complex: application to barley seed encapsulation for improving N uptakeen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1002/jsfa.7466es
dc.identifier.doi10.1002/jsfa.7466
dc.identifier.essn1097-0010
dc.journal.titleJournal of the Science of Food and Agricultureen
dc.volume.number96es
dc.issue.number9es
dc.page.initial2981es
dc.page.final2989es
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record