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dc.contributor.authorOrdóñez Robles, María
dc.contributor.authorSantos Beneit, Fernando
dc.contributor.authorAlbillos García, Silvia María 
dc.contributor.authorLiras, Paloma
dc.contributor.authorMartín, Juan F.
dc.contributor.authorRodríguez García, Antonio
dc.date.accessioned2024-01-11T19:35:21Z
dc.date.available2024-01-11T19:35:21Z
dc.date.issued2017
dc.identifier.issn0175-7598
dc.identifier.urihttp://hdl.handle.net/10259/8308
dc.description.abstractIn this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcriptomic profiling of S. tsukubaensis growing under tacrolimus producing and non-producing conditions. The analysis revealed important and different metabolic changes after the additions and a lack of transcriptional activation of the fkb cluster. In addition, we detected important differences in the transcriptional response to glucose between S. tsukubaensis and the model species Streptomyces coelicolor. A number of genes encoding key players of morphological and biochemical differentiation were strongly and permanently downregulated by the carbon sources. Finally, we identified several genes showing transcriptional profiles highly correlated to that of the tacrolimus biosynthetic pathway regulator FkbN that might be potential candidates for the improvement of tacrolimus production.en
dc.description.sponsorshipThis work was supported by the European Union through an ERA-IB (PIM2010EEI-00677) international cooperation project. M. Ordóñez-Robles received a FPU fellowship of the Ministerio de Educación y Ciencia (Spain).en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherSpringeres
dc.relation.ispartofApplied Microbiology and Biotechnology. 2017, V. 101, n. 22, p. 8181-8195es
dc.subjectStreptomycestsukubaensisen
dc.subjectTacrolimusen
dc.subjectFK506en
dc.subjectCarbon regulationen
dc.subjectTranscriptomicsen
dc.subject.otherMicrobiologíaes
dc.subject.otherMicrobiologyen
dc.subject.otherBiotecnologíaes
dc.subject.otherBiotechnologyen
dc.subject.otherBiologíaes
dc.subject.otherBiologyen
dc.titleStreptomyces tsukubaensis as a new model for carbon repression: transcriptomic response to tacrolimus repressing carbon sourcesen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1007/s00253-017-8545-5es
dc.identifier.doi10.1007/s00253-017-8545-5
dc.identifier.essn1432-0614
dc.journal.titleApplied Microbiology and Biotechnologyen
dc.volume.number101es
dc.issue.number22es
dc.page.initial8181es
dc.page.final8195es
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones


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