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dc.contributor.authorHai, Rong
dc.contributor.authorMartínez Sobrido, Luis
dc.contributor.authorFraser, Kathryn A.
dc.contributor.authorAyllón Barasoain, Juan 
dc.contributor.authorGarcía Sastre, Adolfo
dc.contributor.authorPalese, Peter
dc.date.accessioned2024-01-17T13:11:12Z
dc.date.available2024-01-17T13:11:12Z
dc.date.issued2008-11
dc.identifier.issn0022-538X
dc.identifier.urihttp://hdl.handle.net/10259/8377
dc.description.abstractType B influenza viruses can cause substantial morbidity and mortality in the population, and vaccinationremains by far the best means of protection against infections with these viruses. Here, we report theconstruction of mutant influenza B viruses for potential use as improved live-virus vaccine candidates.Employing reverse genetics, we altered the NS1 gene, which encodes a type I interferon (IFN) antagonist. Theresulting NS1 mutant viruses induced IFN and, as a consequence, were found to be attenuated in vitro and invivo. The absence of pathogenicity of the NS1 mutants in both BALB/c and C57BL/6 PKR / mice wasconfirmed. We also provide evidence that influenza B virus NS1 mutants induce a self-adjuvanted immuneresponse and confer effective protection against challenge with both homologous and heterologous B virusstrains in mice.en
dc.description.sponsorshipWe thank Mark A. Yondola for helpful discussions and criticalreviewing. We also express our appreciation to Christopher Narbus,Lily Ngai, and Richard Cadagan for excellent technical assistance.This work was supported by grants from the NIH, R01 AI46954 (toA.G.-S.), U01 AI70469, and U19 AI62623 (Center for InvestigatingViral Immunity and Antagonism), and by CRIP (Center for Researchon Influenza Pathogenesis, NIAID contract HHSN266200700010C).K.A.F. was supported by NIH training grant T32 AI007647.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Virology. 2008, V. 82, n. 21, p. 10580-10590es
dc.subject.otherMicrobiologíaes
dc.subject.otherMicrobiologyen
dc.subject.otherMedicinaes
dc.subject.otherMedicineen
dc.subject.otherBiología moleculares
dc.subject.otherMolecular biologyen
dc.titleInfluenza B Virus NS1-Truncated Mutants: Live-Attenuated Vaccine Approachen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1128/jvi.01213-08es
dc.identifier.doi10.1128/jvi.01213-08
dc.identifier.essn1098-5514
dc.journal.titleJournal of Virologyen
dc.volume.number82es
dc.issue.number21es
dc.page.initial10580es
dc.page.final10590es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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