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dc.contributor.authorGarcía-Calvo, Laura
dc.contributor.authorRodríguez-Castro, Raquel
dc.contributor.authorUllán, Ricardo V.
dc.contributor.authorAlbillos García, Silvia María 
dc.contributor.authorFernández-Aguado, Marta
dc.contributor.authorVicente, Cláudia M.
dc.contributor.authorDegnes, Kristin F.
dc.contributor.authorSletta, Sletta
dc.contributor.authorBarreiro, Carlos
dc.date.accessioned2024-02-06T09:30:59Z
dc.date.available2024-02-06T09:30:59Z
dc.date.issued2023-02
dc.identifier.issn0175-7598
dc.identifier.urihttp://hdl.handle.net/10259/8596
dc.description.abstractPlant biomass is a promising substrate for biorefinery, as well as a source of bioactive compounds, platform chemicals, and precursors with multiple industrial applications. These applications depend on the hydrolysis of its recalcitrant structure. However, the effective biological degradation of plant cell walls requires several enzymatic groups acting synergistically, and novel enzymes are needed in order to achieve profitable industrial hydrolysis processes. In the present work, a feruloyl esterase (FAE) activity screening of Penicillium spp. strains revealed a promising candidate (Penicillium rubens Wisconsin 54–1255; previously Penicillium chrysogenum), where two FAE-ORFs were identified and subsequently overexpressed. Enzyme extracts were analyzed, confirming the presence of FAE activity in the respective gene products (PrFaeA and PrFaeB). PrFaeB-enriched enzyme extracts were used to determine the FAE activity optima (pH 5.0 and 50–55 °C) and perform proteome analysis by means of MALDI-TOF/TOF mass spectrometry. The studies were completed with the determination of other lignocellulolytic activities, an untargeted metabolite analysis, and upscaled FAE production in stirred tank reactors. The findings described in this work present P. rubens as a promising lignocellulolytic enzyme producer.en
dc.description.sponsorshipThis study was funded by a project (ref. no. CTM2012-32026) of the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) and co-funded by the ERDF (European Regional Development Fund). Ms. Laura García-Calvo was supported by a PhD grant (ref. no. BES-2013–064578) and an international stay grant (ref. no. EEBB-I-16–11760), awarded by the Spanish Ministry MINECO.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherSpringeres
dc.relation.ispartofApplied Microbiology and Biotechnology. 2023, V. 107, n. 2-3, p. 691–717en
dc.subjectFungien
dc.subjectPenicillium rubensen
dc.subjectPenicillium chrysogenumen
dc.subjectLignocelluloseen
dc.subjectFeruloyl esteraseen
dc.subjectExtracellular proteomeen
dc.subject.otherBioquímicaes
dc.subject.otherBiochemistryen
dc.subject.otherBiología moleculares
dc.subject.otherMolecular biologyen
dc.titlePenicillium chrysogenum as a fungal factory for feruloyl esterasesen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1007/s00253-022-12335-wes
dc.identifier.doi10.1007/s00253-022-12335-w
dc.identifier.essn1432-0614
dc.journal.titleApplied Microbiology and Biotechnologyen
dc.volume.number107es
dc.issue.number2-3es
dc.page.initial691es
dc.page.final717es
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones


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