| dc.contributor.author | Ayllón Barasoain, Juan | |
| dc.contributor.author | Hale, Benjamin G. | |
| dc.contributor.author | García Sastre, Adolfo | |
| dc.date.accessioned | 2024-09-04T07:54:51Z | |
| dc.date.available | 2024-09-04T07:54:51Z | |
| dc.date.issued | 2012-05 | |
| dc.identifier.issn | 0022-538X | |
| dc.identifier.uri | http://hdl.handle.net/10259/9525 | |
| dc.description.abstract | We generated influenza A viruses expressing mutant NS1 proteins unable to activate phosphoinositide 3-kinase (PI3K) in two mouse-lethal strains. The recombinant A/Puerto Rico/8/34 (rPR8) mutant virus strain was attenuated and caused reduced morbidity/mortality. For the recombinant A/WSN/33 (rWSN) virus strain, the inability to stimulate PI3K had minimal impact on replication or morbidity/mortality. Cell-based assays revealed subtly distinct intracellular sites of NS1 localization and PI3K activation between the strains. We hypothesize that specific spatially regulated NS1-activated PI3K signaling, rather than simply the total level of active PI3K, is important for virus replication and virulence. | en |
| dc.description.sponsorship | This work was supported by NIH funding to A.G.-S. (Center for Research on Influenza Pathogenesis [CRIP], NIAID CEIRS contract HHSN266200700010C, and NIAID grant RO1AI046954). Confocal laser scanning microscopy was performed at the MSSM-Microscopy Shared Resource Facility, which is supported with funding from a National Institutes of Health-National Cancer Institute (NIH-NCI) shared resources grant (5R24 CA095823-04), an NSF Major Research Instrumentation grant (DBI-9724504), and an NIH shared instrumentation grant (1 S10 RR0 9145-01). | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | eng | es |
| dc.publisher | American Society for Microbiology | en |
| dc.relation.ispartof | Journal of Virology. 2012, V. 86, n. 9, p. 5366–5370 | es |
| dc.subject.other | Medicina | es |
| dc.subject.other | Medicine | en |
| dc.subject.other | Salud | es |
| dc.subject.other | Health | en |
| dc.subject.other | Microbiología | es |
| dc.subject.other | Microbiology | en |
| dc.subject.other | Enfermedades infecciosas | es |
| dc.subject.other | Communicable diseases | en |
| dc.title | Strain-Specific Contribution of NS1-Activated Phosphoinositide 3-Kinase Signaling to Influenza A Virus Replication and Virulence | en |
| dc.type | info:eu-repo/semantics/article | es |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
| dc.relation.publisherversion | https://doi.org/10.1128/jvi.06722-11 | es |
| dc.identifier.doi | 10.1128/jvi.06722-11 | |
| dc.relation.projectID | info:eu-repo/grantAgreement/NIAID//HHSN266200700010C/US/ | es |
| dc.relation.projectID | info:eu-repo/grantAgreement/NIAID//RO1AI046954/US/ | es |
| dc.relation.projectID | info:eu-repo/grantAgreement/NCI//5R24 CA095823-04/US/ | es |
| dc.relation.projectID | info:eu-repo/grantAgreement/NSF//DBI-9724504/US/ | es |
| dc.relation.projectID | info:eu-repo/grantAgreement/NIH//1 S10 RR0 9145-01/US/ | es |
| dc.identifier.essn | 1098-5514 | |
| dc.journal.title | Journal of Virology | en |
| dc.volume.number | 86 | es |
| dc.issue.number | 9 | es |
| dc.page.initial | 5366 | es |
| dc.page.final | 5370 | es |
| dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es |
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