RT info:eu-repo/semantics/article
T1 Analysis of Polycaprolactone Microfibers as Biofilm Carriers for Biotechnologically Relevant Bacteria
A1 Tamayo Ramos, Juan Antonio
A1 Rumbo Lorenzo, Carlos
A1 Caso, Federica
A1 Rinaldi, Antonio
A1 Garroni, Sebastiano
A1 Notargiacomo, Andrea
A1 Romero Santacreu, Lorena
A1 Cuesta López, Santiago
K1 electrospun polycaprolactone
K1 microfibers
K1 biofilm
K1 bacterial attachment
K1 biotechnology
K1 Microbiología
K1 Microbiology
K1 Materiales
K1 Materials
AB Polymeric electrospun fibers are becoming popular in microbial biotechnology because of their exceptional physicochemical characteristics, biodegradability, surface-to-volume ratio, and compatibility with biological systems, which give them a great potential as microbial supports to be used in production processes or environmental applications. In this work, we analyzed and compared the ability of Escherichia coli, Pseudomonas putida, Brevundimonas diminuta, and Sphingobium fuliginis to develop biofilms on different types of polycaprolactone (PCL) microfibers. These bacterial species are relevant in the production of biobased chemicals, enzymes, and proteins for therapeutic use and bioremediation. The obtained results demonstrated that all selected species were able to attach efficiently to the PCL microfibers. Also, the ability of pure cultures of S. fuliginis (former Flavobacterium sp. ATCC 27551, a very relevant strain in the bioremediation of organophosphorus compounds) to form dense biofilms was observed for the first time, opening the possibility of new applications for this microorganism. This material showed to have a high microbial loading capacity, regardless of the mesh density and fiber diameter. A comparative analysis between PCL and polylactic acid (PLA) electrospun microfibers indicated that both surfaces have a similar bacterial loading capacity, but the former material showed higher resistance to microbial degradation than PLA.
PB American Chemical Society
SN 1944-8244
YR 2018
FD 2018-09
LK http://hdl.handle.net/10259/5175
UL http://hdl.handle.net/10259/5175
LA eng
NO European Union’s H2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 691095. The contracts of J.A.T.-R. and C.R. were supported by the grant nos. BU079U16 and BU092U16, that were co-financed by Junta de Castilla y León and the European Social Fund.
DS Repositorio Institucional de la Universidad de Burgos
RD 29-abr-2024