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    Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/10101

    Título
    Inhibitory Properties of Cysteine Protease Pro-Peptides from Barley Confer Resistance to Spider Mite Feeding
    Autor
    Santamaría Fernández, Mª Estrella
    Arnáiz Alonso, AnaAutoridad UBU Orcid
    Díaz Mendoza, Mercedes
    Martínez, Manuel
    Diaz, Isabel
    Publicado en
    PLoS ONE. 2015, V. 10, n. 6, e0128323
    Editorial
    Public Library of Science
    Fecha de publicación
    2015
    DOI
    10.1371/journal.pone.0128323
    Abstract
    C1A plant cysteine proteases are synthesized as pre-pro-enzymes that need to be processed to become active by the pro-peptide claves off from its cognate enzyme. These pro-sequences play multifunctional roles including the capacity to specifically inhibit their own as well as other C1A protease activities from diverse origin. In this study, it is analysed the potential role of C1A pro-regions from barley as regulators of cysteine proteases in target phytophagous arthropods (coleopteran and acari). The in vitro inhibitory action of these pro-sequences, purified as recombinant proteins, is demonstrated. Moreover, transgenic Arabidopsis plants expressing different fragments of HvPap-1 barley gene containing the pro-peptide sequence were generated and the acaricide function was confirmed by bioassays conducted with the two-spotted spider mite Tetranychus urticae. Feeding trials resulted in a significant reduction of leaf damage in the transgenic lines expressing the pro-peptide in comparison to non-transformed control and strongly correlated with an increase in mite mortality. Additionally, the analysis of the expression levels of a selection of potential mite targets (proteases and protease inhibitors) revealed a mite strategy to counteract the inhibitory activity produced by the C1A barley pro-prodomain. These findings demonstrate that pro-peptides can control mite pests and could be applied as defence proteins in biotechnological systems.
    Palabras clave
    Mites
    Genetically modified plants
    Arabidopsis thaliana
    Proteases
    Barley
    Leaves
    Arthropoda
    Protein extraction
    Materia
    Química orgánica
    Chemistry, Organic
    Biotecnología
    Biotechnology
    URI
    http://hdl.handle.net/10259/10101
    Versión del editor
    https://doi.org/10.1371/journal.pone.0128323
    Aparece en las colecciones
    • Artículos POLYMERS
    Atribución 4.0 Internacional
    Documento(s) sujeto(s) a una licencia Creative Commons Atribución 4.0 Internacional
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